| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human PAPPA2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PAPPA2 |
| Gene ID: |
60676 |
| Uniprot ID: |
PAPP2_HUMAN |
| Specificity: |
This kit recognizes Human PAPPA2 in samples. No significant cross-reactivity or interference between Human PAPPA2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed abundantly in placenta, and non-pregnant mammary gland with low expression in the kidney, fetal brain and pancreas. |
| Function | Metalloproteinase which specifically cleaves insulin-like growth factor binding protein (IGFBP)-5 at the '163-Ser-|-Lys-164' bond. Shows limited proteolysis toward IGFBP-3. |
| Protein Name | Pappalysin-2Pregnancy-Associated Plasma Protein A2Papp-A2Pregnancy-Associated Plasma Protein E1Papp-E |
| Database Links | Reactome: R-HSA-381426 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Pappalysin-2 CLIA kitPregnancy-Associated Plasma Protein A2 CLIA kitPapp-A2 CLIA kitPregnancy-Associated Plasma Protein E1 CLIA kitPapp-E CLIA kitPAPPA2 CLIA kitPLAC3 CLIA kit |
| Specificity | This kit recognizes Human PAPPA2 in samples. No significant cross-reactivity or interference between Human PAPPA2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-107 | 101 |
| EDTA plasma(n=8) | 97-109 | 102 |
| Cell culture media(n=8) | 99-116 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 193.10 | 488.02 | 1923.88 | 175.91 | 451.09 | 2052.58 |
| Standard deviation | 19.52 | 37.04 | 190.46 | 14.67 | 42.99 | 189.04 |
| CV (%) | 10.11 | 7.59 | 9.90 | 8.34 | 9.53 | 9.21 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
