| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.23ng/mL |
| Detection Limit: |
0.39~25ng/mL |
| Short Description: |
This OXB Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
1.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
HCRT |
| Gene ID: |
3060 |
| Uniprot ID: |
OREX_HUMAN |
| Specificity: |
This kit recognizes Human OXB in samples. No significant crossreactivity or interference between Human OXB and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | Specific enzymatic cleavages at paired basic residues yield the different active peptides. |
| Function | Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A: Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. Orexin-B: Binds to orexin receptor HCRTR2/OX2R only. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. |
| Protein Name | Hypocretin Neuropeptide PrecursorHypocretinHcrtOrexin PrecursorPrepro-OrexinPreprohypocretin Cleaved Into - Orexin-AHypocretin-1Hcrt1 - Orexin-BHypocretin-2Hcrt2 |
| Database Links | Reactome: R-HSA-389397Reactome: R-HSA-416476 |
| Cellular Localisation | Rough Endoplasmic ReticulumCytoplasmic VesicleSynapseAssociated With Perikaryal Rough Endoplasmic Reticulum As Well As Cytoplasmic Large Granular Vesicles At Synapses |
| Alternative ELISA Names | Hypocretin Neuropeptide Precursor ELISA kitHypocretin ELISA kitHcrt ELISA kitOrexin Precursor ELISA kitPrepro-Orexin ELISA kitPreprohypocretin Cleaved Into - Orexin-A ELISA kitHypocretin-1 ELISA kitHcrt1 - Orexin-B ELISA kitHypocretin-2 ELISA kitHcrt2 ELISA kitHCRT ELISA kitOX ELISA kitPPORX ELISA kitPPOX ELISA kit |
| Specificity | This kit recognizes OXB in samples. No significant cross-reactivity or interference between OXB and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-104 | 95 |
| EDTA plasma(n=8) | 94-110 | 101 |
| Cell culture media(n=8) | 95-109 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.30 | 3.10 | 10.40 | 1.30 | 2.90 | 11.20 |
| Standard deviation | 0.10 | 0.20 | 0.50 | 0.10 | 0.20 | 0.60 |
| CV (%) | 7.69 | 6.45 | 4.81 | 7.69 | 6.90 | 5.36 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
