• Human HCRT (Orexin A) Competitive ELISA (STJE0002658)
  • Human HCRT (Orexin A) Competitive ELISA (STJE0002658)

Human HCRT (Orexin A) Competitive ELISA (STJE0002658)

SKU:
STJE0002658

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This HCRT Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: HCRT
Gene ID: 3060
Uniprot ID: OREX_HUMAN
Specificity: This kit recognizes Human OXA in samples. No significant cross-reactivity or interference between Human OXA and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Specific enzymatic cleavages at paired basic residues yield the different active peptides.
Function Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A: Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. Orexin-B: Binds to orexin receptor HCRTR2/OX2R only. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner.
Protein Name Hypocretin Neuropeptide Precursor
Hypocretin
Hcrt
Orexin Precursor
Prepro-Orexin
Preprohypocretin Cleaved Into - Orexin-A
Hypocretin-1
Hcrt1 - Orexin-B
Hypocretin-2
Hcrt2
Database Links Reactome: R-HSA-389397
Reactome: R-HSA-416476
Cellular Localisation Rough Endoplasmic Reticulum
Cytoplasmic Vesicle
Synapse
Associated With Perikaryal Rough Endoplasmic Reticulum As Well As Cytoplasmic Large Granular Vesicles At Synapses
Alternative ELISA Names Hypocretin Neuropeptide Precursor ELISA kit
Hypocretin ELISA kit
Hcrt ELISA kit
Orexin Precursor ELISA kit
Prepro-Orexin ELISA kit
Preprohypocretin Cleaved Into - Orexin-A ELISA kit
Hypocretin-1 ELISA kit
Hcrt1 - Orexin-B ELISA kit
Hypocretin-2 ELISA kit
Hcrt2 ELISA kit
HCRT ELISA kit
OX ELISA kit
PPORX ELISA kit
PPOX ELISA kit
Specificity This kit recognizes OXA in samples. No significant cross-reactivity or interference between OXA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-106 97
EDTA plasma(n=8) 93-111 101
Cell culture media(n=8) 88-100 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 188.50 568.10 1493.00 170.80 598.40 1389.60
Standard deviation 10.20 25.60 62.70 8.50 31.70 48.60
CV (%) 5.41 4.51 4.20 4.98 5.30 3.50
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance