Name: Human Osteopontin/SPP-1 protein (Recombinant) (C-His) (STJP002678)
SKU: STJP002678
Availability: InStock

General Information
Product Properties
Target Information
Additional Info
Product Review

Host:	HEK293 cells
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Formulation:	Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4.
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method:	< 0.1 EU/Mu g of the protein by LAL method.
Immunoreactivity:	Measured by its binding ability in a functional ELISA. Immobilized Human SPP1 Protein at 1 Mu g/mL (100 Mu L/well) can bind Osteopontin Rabbit mAb with a linear range of 0.976-11.58 ng/mL.

Gene Symbol:	SPP1
Gene ID:	6696
Uniprot ID:	OSTP_HUMAN
Immunogen Region:	Ile17-Asn300
Immunogen:	Recombinant Human Osteopontin/SPP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile17-Asn300) of human Osteopontin (Accession #NP_000573.1) fused with a 6×His tag at the C-terminus.
Immunogen Sequence:	IPVKQADSGSSEEKQLYNKY PDAVATWLNPDPSQKQNLLA PQTLPSKSNESHDHMDDMDD EDDDDHVDSQDSIDSNDSDD VDDTDDSHQSDESHHSDESD ELVTDFPTDLPATEVFTPVV PTVDTYDGRGDSVVYGLRSK SKKFRRPDIQYPDATDEDIT SHMESEELNGAYKAIPVAQD LNAPSDWDSRGKDSYETSQL DDQSAETHSHKQSRLYKRKA NDESNEHSDVIDSQELSKV

Post Translational Modifications	Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.
Function	Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Protein Name	Osteopontin Bone Sialoprotein 1 Nephropontin Secreted Phosphoprotein 1 Spp-1 Urinary Stone Protein Uropontin
Database Links	Reactome: R-HSA-1474228 Reactome: R-HSA-186797 Reactome: R-HSA-216083 Reactome: R-HSA-381426 Reactome: R-HSA-8949275 Reactome: R-HSA-8957275
Cellular Localisation	Secreted
Alternative Protein Names	Osteopontin protein Bone Sialoprotein 1 protein Nephropontin protein Secreted Phosphoprotein 1 protein Spp-1 protein Urinary Stone Protein protein Uropontin protein SPP1 protein BNSP protein OPN protein PSEC0156 protein

Information sourced from Uniprot.org

Model	Name	Host
STJP002436	Mouse Osteopontin/SPP1 protein (Recombinant) (C-His) (STJP002436)	HEK293 cells
STJP002478	Human ENPP-1 protein (Recombinant) (C-His) (STJP002478)	HEK293 cells
STJP001344	Human APLP-1 protein (Recombinant) (C-His) (STJP001344)	HEK293 cells
STJP001006	Human IGFBP-1 protein (Recombinant) (C-His) (STJP001006)	HEK293 cells
STJP001333	Human TIMP-1 protein (Recombinant) (C-His) (STJP001333)	HEK293 cells

Human Osteopontin/SPP-1 protein (Recombinant) (C-His) (STJP002678)

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