Human Osteopontin/SPP-1 protein (Recombinant) (C-His) (STJP002678)

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STJP002678
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Host: HEK293 cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Formulation: Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method: < 0.1 EU/Mu g of the protein by LAL method.
Immunoreactivity: Measured by its binding ability in a functional ELISA. Immobilized Human SPP1 Protein at 1 Mu g/mL (100 Mu L/well) can bind Osteopontin Rabbit mAb with a linear range of 0.976-11.58 ng/mL.
Gene Symbol: SPP1
Gene ID: 6696
Uniprot ID: OSTP_HUMAN
Immunogen Region: Ile17-Asn300
Immunogen: Recombinant Human Osteopontin/SPP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile17-Asn300) of human Osteopontin (Accession #NP_000573.1) fused with a 6×His tag at the C-terminus.
Immunogen Sequence: IPVKQADSGSSEEKQLYNKY PDAVATWLNPDPSQKQNLLA PQTLPSKSNESHDHMDDMDD EDDDDHVDSQDSIDSNDSDD VDDTDDSHQSDESHHSDESD ELVTDFPTDLPATEVFTPVV PTVDTYDGRGDSVVYGLRSK SKKFRRPDIQYPDATDEDIT SHMESEELNGAYKAIPVAQD LNAPSDWDSRGKDSYETSQL DDQSAETHSHKQSRLYKRKA NDESNEHSDVIDSQELSKV
Post Translational Modifications Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.
Function Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Protein Name Osteopontin
Bone Sialoprotein 1
Nephropontin
Secreted Phosphoprotein 1
Spp-1
Urinary Stone Protein
Uropontin
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-186797
Reactome: R-HSA-216083
Reactome: R-HSA-381426
Reactome: R-HSA-8949275
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Protein Names Osteopontin protein
Bone Sialoprotein 1 protein
Nephropontin protein
Secreted Phosphoprotein 1 protein
Spp-1 protein
Urinary Stone Protein protein
Uropontin protein
SPP1 protein
BNSP protein
OPN protein
PSEC0156 protein

Information sourced from Uniprot.org

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