• Human OSM (Oncostatin-M) CLIA Kit (STJC0000927)

Human OSM (Oncostatin-M) CLIA Kit (STJC0000927)

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STJC0000927

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This human OSM kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: OSM
Gene ID: 5008
Uniprot ID: ONCM_HUMAN
Specificity: This kit recognizes Human OSM in samples. No significant cross-reactivity or interference between Human OSM and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Propeptide processing is not important for receptor binding activity but may be important growth-inhibitory activity.
Function Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
Protein Name Oncostatin-M
Osm
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-6788467
Cellular Localisation Secreted
Alternative CLIA Names Oncostatin-M CLIA kit
Osm CLIA kit
OSM CLIA kit
Specificity This kit recognizes Human OSM in samples. No significant cross-reactivity or interference between Human OSM and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-110 103
EDTA plasma(n=8) 91-102 97
Cell culture media(n=8) 95-111 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 27.21 54.57 204.27 28.18 50.28 205.66
Standard deviation 3.22 4.22 18.00 2.86 4.87 12.36
CV (%) 11.83 7.73 8.81 10.15 9.69 6.01
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance