| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88ng/mL |
| Detection Limit: |
3.13~200ng/mL |
| Short Description: |
This OLFM4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
OLFM4 |
| Gene ID: |
10562 |
| Uniprot ID: |
OLFM4_HUMAN |
| Specificity: |
This kit recognizes Human OLFM4 in samples. No significant cross-reactivity or interference between Human OLFM4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated. |
| Function | May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin. |
| Protein Name | Olfactomedin-4Olm4Antiapoptotic Protein Gw112G-Csf-Stimulated Clone 1 ProteinHgc-1Holfd |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | SecretedExtracellular SpaceMitochondrionSubcellular Location Is Not Clearly Defined: Has Been Shown To Be SecretedBut Also In The MitochondrionCytoplasm And Plasma Membrane And In The Nucleus |
| Alternative ELISA Names | Olfactomedin-4 ELISA kitOlm4 ELISA kitAntiapoptotic Protein Gw112 ELISA kitG-Csf-Stimulated Clone 1 Protein ELISA kitHgc-1 ELISA kitHolfd ELISA kitOLFM4 ELISA kitGW112 ELISA kitUNQ362 ELISA kitPRO698 ELISA kit |
| Specificity | This kit recognizes Human OLFM4 in samples. No significant cross-reactivity or interference between Human OLFM4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-96 | 91 |
| EDTA plasma (n=8) | 95-108 | 103 |
| Cell culture media (n=8) | 86-99 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 9.94 | 22.03 | 99.62 | 8.91 | 21.66 | 82.20 |
| Standard deviation | 0.59 | 0.99 | 5.22 | 0.51 | 1.01 | 3.73 |
| CV (%) | 5.94 | 4.49 | 5.24 | 5.72 | 4.66 | 4.54 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
