| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This NOX4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
NOX4 |
| Gene ID: |
50507 |
| Uniprot ID: |
NOX4_HUMAN |
| Specificity: |
This kit recognizes Human NOX4 in samples. No significant cross-reactivity or interference between Human NOX4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function. |
| Function | Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity. Isoform 4: Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1. |
| Protein Name | Nadph Oxidase 4Kidney Oxidase-1Kox-1Kidney Superoxide-Producing Nadph OxidaseRenal Nad(Ph-Oxidase |
| Database Links | Reactome: R-HSA-3299685Reactome: R-HSA-9702518Reactome: R-HSA-9703465 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinCell MembraneCell JunctionFocal AdhesionMay Localize To Plasma Membrane And Focal AdhesionsMay Also Localize To The NucleusIsoform 4: NucleusNucleusNucleolus |
| Alternative ELISA Names | Nadph Oxidase 4 ELISA kitKidney Oxidase-1 ELISA kitKox-1 ELISA kitKidney Superoxide-Producing Nadph Oxidase ELISA kitRenal Nad(Ph-Oxidase ELISA kitNOX4 ELISA kitRENOX ELISA kit |
| Specificity | This kit recognizes Human NOX4 in samples. No significant cross-reactivity or interference between Human NOX4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-102 | 94 |
| EDTA plasma (n=8) | 91-103 | 98 |
| Cell culture media (n=8) | 92-107 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.48 | 1.47 | 4.12 | 0.50 | 1.56 | 3.96 |
| Standard deviation | 0.03 | 0.07 | 0.20 | 0.03 | 0.07 | 0.21 |
| CV (%) | 6.25 | 4.76 | 4.85 | 6.00 | 4.49 | 5.30 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
