| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human NFYC kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
NFYC |
| Gene ID: |
4802 |
| Uniprot ID: |
NFYC_HUMAN |
| Specificity: |
This kit recognizes Human NFYC in samples. No significant cross-reactivity or interference between Human NFYC and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. |
| Protein Name | Nuclear Transcription Factor Y Subunit GammaCaat Box Dna-Binding Protein Subunit CNuclear Transcription Factor Y Subunit CNf-YcTransactivator Hsm-1/2 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-2426168Reactome: R-HSA-380994Reactome: R-HSA-381183Reactome: R-HSA-9614657 |
| Cellular Localisation | Nucleus |
| Alternative CLIA Names | Nuclear Transcription Factor Y Subunit Gamma CLIA kitCaat Box Dna-Binding Protein Subunit C CLIA kitNuclear Transcription Factor Y Subunit C CLIA kitNf-Yc CLIA kitTransactivator Hsm-1/2 CLIA kitNFYC CLIA kit |
| Specificity | This kit recognizes Human NFYC in samples. No significant cross-reactivity or interference between Human NFYC and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 103-114 | 108 |
| EDTA plasma(n=8) | 92-104 | 98 |
| Cell culture media(n=8) | 84-98 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 196.93 | 428.30 | 1860.17 | 199.94 | 397.04 | 1977.06 |
| Standard deviation | 25.56 | 44.97 | 187.88 | 17.01 | 39.51 | 156.78 |
| CV (%) | 12.98 | 10.50 | 10.10 | 8.51 | 9.95 | 7.93 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
