• Human NF2 (Neurofibromin 2) Sandwich ELISA Kit Ready-To-Use (STJE0017318)

Human NF2 (Neurofibromin 2) Sandwich ELISA Kit Ready-To-Use (STJE0017318)

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STJE0017318

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.128ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This NF2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: NF2
Gene ID: 4771
Uniprot ID: MERL_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
Post Translational Modifications Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail. The dephosphorylation of Ser-518 favors the interaction with NOP53. Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation.
Function Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
Protein Name Merlin
Moesin-Ezrin-Radixin-Like Protein
Neurofibromin-2
Schwannomerlin
Schwannomin
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-5627123
Cellular Localisation Isoform 1: Cell Projection
Filopodium Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Ruffle Membrane
Nucleus
In A Fibroblastic Cell Line
Isoform 1 Is Found Homogeneously Distributed Over The Entire Cell
With A Particularly Strong Staining In Ruffling Membranes And Filopodia
Colocalizes With Mpp1 In Non-Myelin-Forming Schwann Cells
Binds With Dcaf1 In The Nucleus
The Intramolecular Association Of The Ferm Domain With The C-Terminal Tail Promotes Nuclear Accumulation
The Unphosphorylated Form Accumulates Predominantly In The Nucleus While The Phosphorylated Form Is Largely Confined To The Non-Nuclear Fractions
Isoform 7: Cytoplasm
Perinuclear Region
Cytoplasmic Granule
Observed In Cytoplasmic Granules Concentrated In A Perinuclear Location
Isoform 7 Is Absent From Ruffling Membranes And Filopodia
Isoform 9: Cytoplasm
Isoform 9 Is Absent From Ruffling Membranes And Filopodia
Isoform 10: Nucleus
Cytoplasm
Cytoskeleton
Isoform 10 Is Found Homogeneously Distributed Over The Entire Cell
Alternative ELISA Names Merlin ELISA kit
Moesin-Ezrin-Radixin-Like Protein ELISA kit
Neurofibromin-2 ELISA kit
Schwannomerlin ELISA kit
Schwannomin ELISA kit
NF2 ELISA kit
SCH ELISA kit
output

Information sourced from Uniprot.org

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