• Human Nesp2 (Nesprin 2) Sandwich ELISA Kit (STJE0010923)

Human Nesp2 (Nesprin 2) Sandwich ELISA Kit (STJE0010923)

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STJE0010923

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.112ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This Nesp2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: SYNE2
Gene ID: 23224
Uniprot ID: SYNE2_HUMAN
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity Widely expressed, with higher level in kidney, adult and fetal liver, stomach and placenta. Weakly expressed in skeletal muscle and brain. Isoform 5 is highly expressed in pancreas, skeletal muscle and heart.
Post Translational Modifications The disulfid bond with SUN2 is required for stability of the SUN2:SYNE2/KASH2 LINC complex under tensile forces though not required for the interaction. Ubiquitinated, targeting it for degradation.
Function Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probable association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome.SYNE1 and SYNE2 may act redundantly. During INM at G1 phase mediates respective LINC complex association with kinesin to push the nucleus away from the centrosome. Involved in nuclear migration in retinal photoreceptor progenitors. Required for centrosome migration to the apical cell surface during early ciliogenesis. Facilitates the relaxation of mechanical stress imposed by compressive actin fibers at the rupture site through its nteraction with SYN2.
Protein Name Nesprin-2
Kash Domain-Containing Protein 2
Kash2
Nuclear Envelope Spectrin Repeat Protein 2
Nucleus And Actin Connecting Element Protein
Protein Nuance
Synaptic Nuclear Envelope Protein 2
Syne-2
Database Links Reactome: R-HSA-1221632
Cellular Localisation Nucleus Outer Membrane
Single-Pass Type Iv Membrane Protein
Cytoplasmic Side
Sarcoplasmic Reticulum Membrane
Cell Membrane
Single-Pass Membrane Protein
Cytoplasm
Cytoskeleton
Mitochondrion
Nucleus
Nucleoplasm
Myofibril
Sarcomere
Z Line
Different Isoform Patterns Are Found In The Different Compartments Of The Cell
The Isoforms Having The C-Terminal Transmembrane Span Can Be Found In Several Organellar Membranes Like The Nuclear Envelope
The Sarcoplasmic Reticulum Of Myoblasts
Or The Lamellipodia And Focal Adhesions At The Cell Membrane
The Largest Part Of The Outer Nuclear Membrane-Associated Protein Is Cytoplasmic
While Its C-Terminal Part Is Associated With The Nuclear Envelope
Most Probably The Outer Nuclear Membrane
Remains Associated With The Nuclear Envelope During Its Breakdown In Mitotic Cells
Shorter Soluble Isoforms Can Be Found In The Cytoplasm And Within The Nucleus
Isoform 8: Cell Junction
Focal Adhesion
In U2os Cells
Alternative ELISA Names Nesprin-2 ELISA kit
Kash Domain-Containing Protein 2 ELISA kit
Kash2 ELISA kit
Nuclear Envelope Spectrin Repeat Protein 2 ELISA kit
Nucleus And Actin Connecting Element Protein ELISA kit
Protein Nuance ELISA kit
Synaptic Nuclear Envelope Protein 2 ELISA kit
Syne-2 ELISA kit
SYNE2 ELISA kit
KIAA1011 ELISA kit
NUA ELISA kit
output

Information sourced from Uniprot.org

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