• Human MUC7 (Mucin-7) Sandwich ELISA (STJE0003587)

Human MUC7 (Mucin-7) Sandwich ELISA (STJE0003587)

SKU:
STJE0003587

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This MUC7 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: MUC7
Gene ID: 4589
Uniprot ID: MUC7_HUMAN
Specificity: This kit recognizes Human MUC7 in samples. No significant cross-reactivity or interference between Human MUC7 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N- and O-glycosylated. Contains fucose, mannose, galactose, N-acetylglucosamine and N-acetylgalactosamine.
Function May function in a protective capacity by promoting the clearance of bacteria in the oral cavity and aiding in mastication, speech, and swallowing. Binds P.aeruginosa pili.
Protein Name Mucin-7
Muc-7
Apo-Mg2
Salivary Mucin-7
Database Links Reactome: R-HSA-5083625
Reactome: R-HSA-5083632
Reactome: R-HSA-5083636
Reactome: R-HSA-5621480
Reactome: R-HSA-913709
Reactome: R-HSA-977068
Cellular Localisation Secreted
Alternative ELISA Names Mucin-7 ELISA kit
Muc-7 ELISA kit
Apo-Mg2 ELISA kit
Salivary Mucin-7 ELISA kit
MUC7 ELISA kit
MG2 ELISA kit
Specificity This kit recognizes Human MUC7 in samples. No significant cross-reactivity or interference between Human MUC7 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 95
EDTA plasma (n=8) 89-103 96
Cell culture media (n=8) 87-99 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.10 2.41 8.67 1.14 2.44 8.90
Standard deviation 0.06 0.11 0.36 0.06 0.14 0.29
CV (%) 5.45 4.56 4.15 5.26 5.74 3.26
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance