| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 37.5pg/mL |
| Detection Limit: | 62.5~4000pg/mL |
| Short Description : | This human MUC4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | MUC4 |
| Gene ID: | 4585 |
| Uniprot ID: | MUC4_HUMAN |
| Specificity: | This kit recognizes Human MUC4 in samples. No significant cross-reactivity or interference between Human MUC4 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines. |
| Post Translational Modifications | Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain. Mucin-4 alpha chain: Highly O-glycosylated. Mucin-4 beta chain: Is predominantly N-glycosylated. |
| Function | Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces. These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation. Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. |
| Protein Name | Mucin-4 Muc-4 Ascites Sialoglycoprotein Asgp Pancreatic Adenocarcinoma Mucin Testis Mucin Tracheobronchial Mucin Cleaved Into - Mucin-4 Alpha Chain Ascites Sialoglycoprotein 1 Asgp-1 - Mucin-4 Beta Chain Ascites Sialoglycoprotein 2 Asgp-2 |
| Database Links | Reactome: R-HSA-5083625 Reactome: R-HSA-5083632 Reactome: R-HSA-5083636 Reactome: R-HSA-5621480 Reactome: R-HSA-913709 Reactome: R-HSA-977068 |
| Cellular Localisation | Mucin-4 Beta Chain: Cell Membrane Single-Pass Membrane Protein Isoforms Lacking The Cys-Rich Region Egf-Like Domains And Transmembrane Region Are Secreted Secretion Occurs By Splicing Or Proteolytic Processing Mucin-4 Alpha Chain: Cell Membrane Secreted Forms A Complex With Mucin-4 Beta Chain At The Cell Membrane Isoform 3: Cell Membrane Isoform 11: Secreted Isoform 15: Secreted Isoform 17: Cell Membrane |
| Alternative CLIA Names | Mucin-4 CLIA kit Muc-4 CLIA kit Ascites Sialoglycoprotein CLIA kit Asgp CLIA kit Pancreatic Adenocarcinoma Mucin CLIA kit Testis Mucin CLIA kit Tracheobronchial Mucin Cleaved Into - Mucin-4 Alpha Chain CLIA kit Ascites Sialoglycoprotein 1 CLIA kit Asgp-1 - Mucin-4 Beta Chain CLIA kit Ascites Sialoglycoprotein 2 CLIA kit Asgp-2 CLIA kit MUC4 CLIA kit |
| Specificity | This kit recognizes Human MUC4 in samples. No significant cross-reactivity or interference between Human MUC4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 90-105 | 98 |
| EDTA plasma(n=8) | 85-97 | 91 |
| Cell culture media(n=8) | 90-104 | 98 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 204.66 | 626.41 | 1420.50 | 209.12 | 675.43 | 1549.06 |
| Standard deviation | 24.29 | 48.11 | 105.69 | 20.79 | 62.27 | 128.73 |
| CV (%) | 11.87 | 7.68 | 7.44 | 9.94 | 9.22 | 8.31 |