| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human MSMB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MSMB |
| Gene ID: |
4477 |
| Uniprot ID: |
MSMB_HUMAN |
| Specificity: |
This kit recognizes Human MSM Beta in samples. No significant cross-reactivity or interference between Human MSM Beta and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Strongly expressed in prostate, liver, kidney, breast and penis. Also expressed in pancreas, esophagus, stomach, deodenum, colon, trachea, lung, salivary glands and fallopian tube. PSP94 is expressed in lung and breast, whereas PSP57 is found in kidney and bladder. |
| Protein Name | Beta-MicroseminoproteinImmunoglobulin-Binding FactorIgbfPn44Prostate Secreted Seminal Plasma ProteinProstate Secretory Protein Of 94 Amino AcidsPsp-94Psp94Seminal Plasma Beta-Inhibin |
| Cellular Localisation | SecretedSperm Surface |
| Alternative CLIA Names | Beta-Microseminoprotein CLIA kitImmunoglobulin-Binding Factor CLIA kitIgbf CLIA kitPn44 CLIA kitProstate Secreted Seminal Plasma Protein CLIA kitProstate Secretory Protein Of 94 Amino Acids CLIA kitPsp-94 CLIA kitPsp94 CLIA kitSeminal Plasma Beta-Inhibin CLIA kitMSMB CLIA kitPRSP CLIA kit |
| Specificity | This kit recognizes Human MSMβ in samples. No significant cross-reactivity or interference between Human MSMβ and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-101 | 95 |
| EDTA plasma(n=8) | 97-112 | 105 |
| Cell culture media(n=8) | 94-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 38.07 | 75.59 | 297.62 | 37.22 | 81.64 | 312.54 |
| Standard deviation | 4.79 | 5.62 | 24.49 | 3.67 | 6.50 | 30.66 |
| CV (%) | 12.58 | 7.43 | 8.23 | 9.86 | 7.96 | 9.81 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
