| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human MMP24 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MMP24 |
| Gene ID: |
10893 |
| Uniprot ID: |
MMP24_HUMAN |
| Specificity: |
This kit recognizes Human MMP-24 in samples. No significant cross-reactivity or interference between Human MMP-24 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Predominantly expressed in brain, kidney, pancreas and lung. Overexpressed in a series of brain tumors, including astrocytomas and glioblastomas. |
| Post Translational Modifications | Cleaved by a furin endopeptidase in the trans-Golgi network. |
| Function | Metalloprotease that mediates cleavage of N-cadherin (CDH2) and acts as a regulator of neuro-immune interactions and neural stem cell quiescence. Involved in cell-cell interactions between nociceptive neurites and mast cells, possibly by mediating cleavage of CDH2, thereby acting as a mediator of peripheral thermal nociception and inflammatory hyperalgesia. Key regulator of neural stem cells quiescence by mediating cleavage of CDH2, affecting CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate their quiescence. May play a role in axonal growth. Able to activate progelatinase A. May also be a proteoglycanase involved in degradation of proteoglycans, such as dermatan sulfate and chondroitin sulfate proteoglycans. Cleaves partially fibronectin, but not collagen type I, nor laminin. |
| Protein Name | Matrix Metalloproteinase-24Mmp-24Membrane-Type Matrix Metalloproteinase 5Mt-Mmp 5Mtmmp5Membrane-Type-5 Matrix MetalloproteinaseMt5-MmpMt5mmp Cleaved Into - Processed Matrix Metalloproteinase-24 |
| Database Links | Reactome: R-HSA-1592389 |
| Cellular Localisation | Matrix Metalloproteinase-24: Cell MembraneSingle-Pass Type I Membrane ProteinGolgi ApparatusTrans-Golgi Network MembraneRecycled Back To The Plasma Membrane Through The Trans-Golgi Network Via Interaction With Apba3Processed Matrix Metalloproteinase-24: SecretedExtracellular SpaceExtracellular MatrixAlso Shed From Cell Surface As Soluble ProteinaseBy A Proteolytic Cleavage |
| Alternative CLIA Names | Matrix Metalloproteinase-24 CLIA kitMmp-24 CLIA kitMembrane-Type Matrix Metalloproteinase 5 CLIA kitMt-Mmp 5 CLIA kitMtmmp5 CLIA kitMembrane-Type-5 Matrix Metalloproteinase CLIA kitMt5-Mmp CLIA kitMt5mmp Cleaved Into - Processed Matrix Metalloproteinase-24 CLIA kitMMP24 CLIA kitMT5MMP CLIA kit |
| Specificity | This kit recognizes Human MMP-24 in samples. No significant cross-reactivity or interference between Human MMP-24 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 98-114 | 106 |
| EDTA plasma(n=8) | 89-103 | 95 |
| Cell culture media(n=8) | 89-106 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 107.49 | 205.28 | 774.76 | 112.84 | 191.68 | 757.06 |
| Standard deviation | 11.30 | 20.75 | 59.19 | 14.09 | 16.18 | 65.64 |
| CV (%) | 10.51 | 10.11 | 7.64 | 12.49 | 8.44 | 8.67 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
