• Human MMP12 (Macrophage metalloelastase) Sandwich ELISA (STJE0002008)

Human MMP12 (Macrophage metalloelastase) Sandwich ELISA (STJE0002008)

SKU:
STJE0002008

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This MMP12 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: MMP12
Gene ID: 4321
Uniprot ID: MMP12_HUMAN
Specificity: This kit recognizes Human MMP-12 in samples. No significant cross-reactivity or interference between Human MMP-12 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Protein Name Macrophage Metalloelastase
Mme
Macrophage Elastase
Me
Hme
Matrix Metalloproteinase-12
Mmp-12
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative ELISA Names Macrophage Metalloelastase ELISA kit
Mme ELISA kit
Macrophage Elastase ELISA kit
Me ELISA kit
Hme ELISA kit
Matrix Metalloproteinase-12 ELISA kit
Mmp-12 ELISA kit
MMP12 ELISA kit
HME ELISA kit
Specificity This kit recognizes Human MMP-12 in samples. No significant cross-reactivity or interference between Human MMP-12 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-109 101
EDTA plasma (n=8) 93-108 99
Cell culture media (n=8) 92-106 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.09 2.01 8.16 1.15 1.94 8.53
Standard deviation 0.06 0.11 0.41 0.07 0.11 0.31
CV (%) 5.50 5.47 5.02 6.09 5.67 3.63
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance