• Human MMP11 (Stromelysin-3) CLIA Kit (STJC0000644)

Human MMP11 (Stromelysin-3) CLIA Kit (STJC0000644)

SKU:
STJC0000644

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human MMP11 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: MMP11
Gene ID: 4320
Uniprot ID: MMP11_HUMAN
Specificity: This kit recognizes Human MMP-11 in samples. No significant cross-reactivity or interference between Human MMP-11 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Specifically expressed in stromal cells of breast carcinomas.
Post Translational Modifications The precursor is cleaved by a furin endopeptidase.
Function May play an important role in the progression of epithelial malignancies.
Protein Name Stromelysin-3
Sl-3
St3
Matrix Metalloproteinase-11
Mmp-11
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative CLIA Names Stromelysin-3 CLIA kit
Sl-3 CLIA kit
St3 CLIA kit
Matrix Metalloproteinase-11 CLIA kit
Mmp-11 CLIA kit
MMP11 CLIA kit
STMY3 CLIA kit
Specificity This kit recognizes Human MMP-11 in samples. No significant cross-reactivity or interference between Human MMP-11 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-111 104
EDTA plasma(n=8) 88-102 94
Cell culture media(n=8) 100-113 107

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 110.56 282.34 841.22 116.47 301.58 826.08
Standard deviation 12.25 32.84 82.27 9.67 35.19 65.34
CV (%) 11.08 11.63 9.78 8.30 11.67 7.91
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance