| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human MMP11 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
MMP11 |
| Gene ID: |
4320 |
| Uniprot ID: |
MMP11_HUMAN |
| Specificity: |
This kit recognizes Human MMP-11 in samples. No significant cross-reactivity or interference between Human MMP-11 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Specifically expressed in stromal cells of breast carcinomas. |
| Post Translational Modifications | The precursor is cleaved by a furin endopeptidase. |
| Function | May play an important role in the progression of epithelial malignancies. |
| Protein Name | Stromelysin-3Sl-3St3Matrix Metalloproteinase-11Mmp-11 |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Stromelysin-3 CLIA kitSl-3 CLIA kitSt3 CLIA kitMatrix Metalloproteinase-11 CLIA kitMmp-11 CLIA kitMMP11 CLIA kitSTMY3 CLIA kit |
| Specificity | This kit recognizes Human MMP-11 in samples. No significant cross-reactivity or interference between Human MMP-11 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-111 | 104 |
| EDTA plasma(n=8) | 88-102 | 94 |
| Cell culture media(n=8) | 100-113 | 107 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 110.56 | 282.34 | 841.22 | 116.47 | 301.58 | 826.08 |
| Standard deviation | 12.25 | 32.84 | 82.27 | 9.67 | 35.19 | 65.34 |
| CV (%) | 11.08 | 11.63 | 9.78 | 8.30 | 11.67 | 7.91 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
