| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This MKI67 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
MKI67 |
| Gene ID: |
4288 |
| Uniprot ID: |
KI67_HUMAN |
| Specificity: |
This kit recognizes Human Ki67P in samples. No significant cross-reactivity or interference between Human Ki67P and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). |
| Protein Name | Proliferation Marker Protein Ki-67Antigen Identified By Monoclonal Antibody Ki-67Antigen Ki-67Antigen Ki67 |
| Cellular Localisation | ChromosomeNucleusNucleolusAssociates With The Surface Of The Mitotic ChromosomeThe Perichromosomal LayerAnd Covers A Substantial Fraction Of The Mitotic Chromosome SurfaceAssociates With Satellite Dna In G1 PhaseBinds Tightly To Chromatin In InterphaseChromatin-Binding Decreases In Mitosis When It Associates With The Surface Of The Condensed ChromosomesPredominantly Localized In The G1 Phase In The Perinucleolar RegionIn The Later Phases It Is Also Detected Throughout The Nuclear InteriorBeing Predominantly Localized In The Nuclear Matrix |
| Alternative ELISA Names | Proliferation Marker Protein Ki-67 ELISA kitAntigen Identified By Monoclonal Antibody Ki-67 ELISA kitAntigen Ki-67 ELISA kitAntigen Ki67 ELISA kitMKI67 ELISA kit |
| Specificity | This kit recognizes Human Ki67P in samples. No significant cross-reactivity or interference between Human Ki67P and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-105 | 96 |
| EDTA plasma (n=8) | 95-109 | 102 |
| Cell culture media (n=8) | 91-107 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.92 | 3.10 | 9.40 | 0.83 | 3.26 | 8.87 |
| Standard deviation | 0.05 | 0.13 | 0.49 | 0.04 | 0.17 | 0.38 |
| CV (%) | 5.43 | 4.19 | 5.21 | 4.82 | 5.21 | 4.28 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
