| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.04 pg/mL |
| Detection Limit: |
7.81-500 pg/mL |
| Short Description: |
This MIP-1 Beta Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CCL4 |
| Gene ID: |
388372/6351 |
| Uniprot ID: |
CCL4_HUMAN |
| Specificity: |
This kit recognizes Human MIP-1 Beta in samples. No significant cross-reactivity or interference between Human MIP-1 Beta and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Post Translational Modifications | N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. |
| Function | Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. |
| Protein Name | C-C Motif Chemokine 4G-26 T-Lymphocyte-Secreted ProteinHc21Lymphocyte Activation Gene 1 ProteinLag-1Mip-1-Beta(1-69Macrophage Inflammatory Protein 1-BetaMip-1-BetaPat 744Protein H400Sis-GammaSmall-Inducible Cytokine A4T-Cell Activation Protein 2Act-2 Cleaved Into - Mip-1-Beta(3-69 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-6783783 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | C-C Motif Chemokine 4 ELISA kitG-26 T-Lymphocyte-Secreted Protein ELISA kitHc21 ELISA kitLymphocyte Activation Gene 1 Protein ELISA kitLag-1 ELISA kitMip-1-Beta(1-69 ELISA kitMacrophage Inflammatory Protein 1-Beta ELISA kitMip-1-Beta ELISA kitPat 744 ELISA kitProtein H400 ELISA kitSis-Gamma ELISA kitSmall-Inducible Cytokine A4 ELISA kitT-Cell Activation Protein 2 ELISA kitAct-2 Cleaved Into - Mip-1-Beta(3-69 ELISA kitCCL4 ELISA kitLAG1 ELISA kitMIP1B ELISA kitSCYA4 ELISA kit |
| Specificity | This kit recognizes Human MIP-1β in samples. No significant cross-reactivity or interference between Human MIP-1β and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-102 | 94 |
| EDTA plasma (n=8) | 90-105 | 97 |
| Cell culture media (n=8) | 90-100 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 22.84 | 59.65 | 199.49 | 20.60 | 56.38 | 190.25 |
| Standard deviation | 1.59 | 3.29 | 6.80 | 1.12 | 2.31 | 9.36 |
| CV (%) | 6.94 | 5.51 | 3.41 | 5.44 | 4.10 | 4.92 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
