| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.62 pg/mL |
| Detection Limit: |
3.13-200 pg/mL |
| Short Description: |
This MIP-1 Alpha Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CCL3 |
| Gene ID: |
6348 |
| Uniprot ID: |
CCL3_HUMAN |
| Specificity: |
This kit recognizes Human MIP-1 Alpha in samples. No significant cross-reactivity or interference between Human MIP-1 Alpha and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Post Translational Modifications | N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells. |
| Function | Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). |
| Protein Name | C-C Motif Chemokine 3G0/G1 Switch Regulatory Protein 19-1Macrophage Inflammatory Protein 1-AlphaMip-1-AlphaPat 464.1Sis-BetaSmall-Inducible Cytokine A3Tonsillar Lymphocyte Ld78 Alpha Protein Cleaved Into - Mip-1-Alpha(4-69Ld78-Alpha(4-69 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-6783783 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | C-C Motif Chemokine 3 ELISA kitG0/G1 Switch Regulatory Protein 19-1 ELISA kitMacrophage Inflammatory Protein 1-Alpha ELISA kitMip-1-Alpha ELISA kitPat 464.1 ELISA kitSis-Beta ELISA kitSmall-Inducible Cytokine A3 ELISA kitTonsillar Lymphocyte Ld78 Alpha Protein Cleaved Into - Mip-1-Alpha(4-69 ELISA kitLd78-Alpha(4-69 ELISA kitCCL3 ELISA kitG0S19-1 ELISA kitMIP1A ELISA kitSCYA3 ELISA kit |
| Specificity | This kit recognizes Human MIP-1α in samples. No significant cross-reactivity or interference between Human MIP-1α and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-104 | 95 |
| EDTA plasma (n=8) | 94-108 | 99 |
| Cell culture media (n=8) | 91-103 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 10.25 | 20.84 | 78.04 | 10.29 | 22.66 | 72.89 |
| Standard deviation | 0.53 | 1.03 | 2.81 | 0.65 | 1.21 | 3.00 |
| CV (%) | 5.18 | 4.94 | 3.60 | 6.36 | 5.36 | 4.11 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
