| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This MAP-2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
METAP2 |
| Gene ID: |
10988 |
| Uniprot ID: |
MAP2_HUMAN |
| Sample Type: |
serum, plasma and other biological fluids |
| Function | Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. |
| Protein Name | Methionine Aminopeptidase 2Map 2Metap 2Initiation Factor 2-Associated 67 Kda GlycoproteinP67P67eif2Peptidase M |
| Database Links | Reactome: R-HSA-2514859 |
| Cellular Localisation | CytoplasmAbout 30% Of Expressed Metap2 Associates With Polysomes |
| Alternative ELISA Names | Methionine Aminopeptidase 2 ELISA kitMap 2 ELISA kitMetap 2 ELISA kitInitiation Factor 2-Associated 67 Kda Glycoprotein ELISA kitP67 ELISA kitP67eif2 ELISA kitPeptidase M ELISA kitMETAP2 ELISA kitMNPEP ELISA kitP67EIF2 ELISA kit |
| Specificity | This kit recognizes Human MAP-2 in samples. No significant cross-reactivity or interference between Human MAP-2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-106 | 100 |
| EDTA plasma (n=8) | 86-98 | 91 |
| Cell culture media (n=8) | 92-106 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.05 | 2.09 | 8.34 | 1.00 | 2.26 | 8.79 |
| Standard deviation | 0.07 | 0.10 | 0.43 | 0.06 | 0.13 | 0.45 |
| CV (%) | 6.67 | 4.78 | 5.16 | 6.00 | 5.75 | 5.12 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
