| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
1.29ng/mL |
| Detection Limit: |
3.12-200ng/mL |
| Short Description: |
This LPCAT3 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
LPCAT3 |
| Gene ID: |
10162 |
| Uniprot ID: |
MBOA5_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Highly expressed in liver, pancreas and adipose tissue. Very low expression in skeletal muscle and heart. Detected in neutrophils. |
| Post Translational Modifications | |
| Function | Lysophospholipid O-acyltransferase (LPLAT) that catalyzes the reacylation step of the phospholipid remodeling process also known as the Lands cycle. Catalyzes transfer of the fatty acyl chain from fatty acyl-CoA to 1-acyl lysophospholipid to form various classes of phospholipids. Converts 1-acyl lysophosphatidylcholine (LPC) into phosphatidylcholine (PC) (LPCAT activity), 1-acyl lysophosphatidylserine (LPS) into phosphatidylserine (PS) (LPSAT activity) and 1-acyl lysophosphatidylethanolamine (LPE) into phosphatidylethanolamine (PE) (LPEAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Has higher activity for LPC acyl acceptors compared to LPEs and LPSs. Can also transfer the fatty acyl chain from fatty acyl-CoA to 1-O-alkyl lysophospholipid or 1-O-alkenyl lysophospholipid with lower efficiency. Acts as a major LPC O-acyltransferase in liver and intestine. As a component of the liver X receptor/NR1H3 or NR1H2 signaling pathway, mainly catalyzes the incorporation of arachidonate into PCs of endoplasmic reticulum (ER) membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Promotes processing of sterol regulatory protein SREBF1 in hepatocytes, likely by facilitating the translocation of SREBF1-SCAP complex from ER to the Golgi apparatus. Participates in mechanisms by which the liver X receptor/NR1H3 or NR1H2 signaling pathway counteracts lipid-induced ER stress response and inflammation. Down-regulates hepatic inflammation by limiting arachidonic acid availability for synthesis of inflammatory eicosanoids, such as prostaglandins. In enterocytes, acts as a component of a gut-brain feedback loop that coordinates dietary lipid absorption and food intake. Regulates the abundance of PCs containing linoleate and arachidonate in enterocyte membranes, enabling passive diffusion of fatty acids and cholesterol across the membrane for efficient chylomicron assembly. In the intestinal crypt, acts as a component of dietary-responsive phospholipid-cholesterol axis, regulating the biosynthesis of cholesterol and its mitogenic effects on intestinal stem cells. |
| Protein Name | Lysophospholipid Acyltransferase 5Lplat 51-Acylglycerophosphocholine O-Acyltransferase1-Acylglycerophosphoethanolamine O-Acyltransferase1-Acylglycerophosphoserine O-AcyltransferaseLysophosphatidylcholine AcyltransferaseLpcatLyso-Pc AcyltransferaseLysophosphatidylcholine Acyltransferase 3Lyso-Pc Acyltransferase 3Lysophosphatidylserine AcyltransferaseLpsatLyso-Ps AcyltransferaseMembrane-Bound O-Acyltransferase Domain-Containing Protein 5O-Acyltransferase Domain-Containing Protein 5 |
| Database Links | Reactome: R-HSA-1482788Reactome: R-HSA-1482801Reactome: R-HSA-1482839 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative ELISA Names | Lysophospholipid Acyltransferase 5 ELISA kitLplat 5 ELISA kit1-Acylglycerophosphocholine O-Acyltransferase ELISA kit1-Acylglycerophosphoethanolamine O-Acyltransferase ELISA kit1-Acylglycerophosphoserine O-Acyltransferase ELISA kitLysophosphatidylcholine Acyltransferase ELISA kitLpcat ELISA kitLyso-Pc Acyltransferase ELISA kitLysophosphatidylcholine Acyltransferase 3 ELISA kitLyso-Pc Acyltransferase 3 ELISA kitLysophosphatidylserine Acyltransferase ELISA kitLpsat ELISA kitLyso-Ps Acyltransferase ELISA kitMembrane-Bound O-Acyltransferase Domain-Containing Protein 5 ELISA kitO-Acyltransferase Domain-Containing Protein 5 ELISA kitLPCAT3 ELISA kitMBOAT5 ELISA kitOACT5 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
