| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human LOXL4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
LOXL4 |
| Gene ID: |
84171 |
| Uniprot ID: |
LOXL4_HUMAN |
| Specificity: |
This kit recognizes Human LOXL4 in samples. No significant cross-reactivity or interference between Human LOXL4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in many tissues, the highest levels among the tissues studied being in the skeletal muscle, testis and pancreas. Expressed in cartilage. |
| Post Translational Modifications | The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. |
| Function | May modulate the formation of a collagenous extracellular matrix. |
| Protein Name | Lysyl Oxidase Homolog 4Lysyl Oxidase-Like Protein 4Lysyl Oxidase-Related Protein C |
| Database Links | Reactome: R-HSA-1566948Reactome: R-HSA-2243919 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative CLIA Names | Lysyl Oxidase Homolog 4 CLIA kitLysyl Oxidase-Like Protein 4 CLIA kitLysyl Oxidase-Related Protein C CLIA kitLOXL4 CLIA kitLOXC CLIA kit |
| Specificity | This kit recognizes Human LOXL4 in samples. No significant cross-reactivity or interference between Human LOXL4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 97-111 | 105 |
| EDTA plasma(n=8) | 91-106 | 97 |
| Cell culture media(n=8) | 102-117 | 108 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 206.00 | 440.71 | 1829.87 | 222.42 | 418.65 | 1930.02 |
| Standard deviation | 16.87 | 46.63 | 118.58 | 22.69 | 39.06 | 196.09 |
| CV (%) | 8.19 | 10.58 | 6.48 | 10.20 | 9.33 | 10.16 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
