| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This LOX Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
LOX |
| Gene ID: |
4015 |
| Uniprot ID: |
LYOX_HUMAN |
| Specificity: |
This kit recognizes Human LOX in samples. No significant cross-reactivity or interference between Human LOX and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. Proteolytically cleaved by BMP1 which removes the propeptide. Also proteolytically cleaved by ADAMTS2 and ADAMTS14, but not by ADAMTS3, at an additional cleavage site downstream of the BMP1 cleavage site. The propeptide plays a role in directing the deposition of this enzyme to elastic fibers, via interaction with tropoelastin. Cleavage by BMP1 to remove the propeptide does not increase enzymatic activity but increases binding to collagen. Cleavage by ADAMTS2 produces a form with reduced collagen-binding activity. Sulfated at Tyr-187 and also at either Tyr-183 or Tyr-184 which enhances binding to collagen. |
| Function | Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture. |
| Protein Name | Protein-Lysine 6-OxidaseLysyl Oxidase Cleaved Into - Protein-Lysine 6-Oxidase - Long Form - Protein-Lysine 6-Oxidase - Short Form |
| Database Links | Reactome: R-HSA-1566948Reactome: R-HSA-2243919 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative ELISA Names | Protein-Lysine 6-Oxidase ELISA kitLysyl Oxidase Cleaved Into - Protein-Lysine 6-Oxidase - Long Form - Protein-Lysine 6-Oxidase - Short Form ELISA kitLOX ELISA kit |
| Specificity | This kit recognizes Human LOX in samples. No significant cross-reactivity or interference between Human LOX and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-104 | 99 |
| EDTA plasma (n=8) | 87-100 | 93 |
| Cell culture media (n=8) | 93-111 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.96 | 4.41 | 16.22 | 2.09 | 4.47 | 16.14 |
| Standard deviation | 0.11 | 0.24 | 0.56 | 0.12 | 0.19 | 0.54 |
| CV (%) | 5.61 | 5.44 | 3.45 | 5.74 | 4.25 | 3.35 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
