• Human LOX (Protein-lysine 6-oxidase) Sandwich ELISA (STJE0002032)

Human LOX (Protein-lysine 6-oxidase) Sandwich ELISA (STJE0002032)

SKU:
STJE0002032

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.38ng/mL
Detection Limit: 0.63~40ng/mL
Short Description: This LOX Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: LOX
Gene ID: 4015
Uniprot ID: LYOX_HUMAN
Specificity: This kit recognizes Human LOX in samples. No significant cross-reactivity or interference between Human LOX and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. Proteolytically cleaved by BMP1 which removes the propeptide. Also proteolytically cleaved by ADAMTS2 and ADAMTS14, but not by ADAMTS3, at an additional cleavage site downstream of the BMP1 cleavage site. The propeptide plays a role in directing the deposition of this enzyme to elastic fibers, via interaction with tropoelastin. Cleavage by BMP1 to remove the propeptide does not increase enzymatic activity but increases binding to collagen. Cleavage by ADAMTS2 produces a form with reduced collagen-binding activity. Sulfated at Tyr-187 and also at either Tyr-183 or Tyr-184 which enhances binding to collagen.
Function Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture.
Protein Name Protein-Lysine 6-Oxidase
Lysyl Oxidase Cleaved Into - Protein-Lysine 6-Oxidase - Long Form - Protein-Lysine 6-Oxidase - Short Form
Database Links Reactome: R-HSA-1566948
Reactome: R-HSA-2243919
Cellular Localisation Secreted
Extracellular Space
Alternative ELISA Names Protein-Lysine 6-Oxidase ELISA kit
Lysyl Oxidase Cleaved Into - Protein-Lysine 6-Oxidase - Long Form - Protein-Lysine 6-Oxidase - Short Form ELISA kit
LOX ELISA kit
Specificity This kit recognizes Human LOX in samples. No significant cross-reactivity or interference between Human LOX and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-104 99
EDTA plasma (n=8) 87-100 93
Cell culture media (n=8) 93-111 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.96 4.41 16.22 2.09 4.47 16.14
Standard deviation 0.11 0.24 0.56 0.12 0.19 0.54
CV (%) 5.61 5.44 3.45 5.74 4.25 3.35
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance