| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human LIF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
LIF |
| Gene ID: |
3976 |
| Uniprot ID: |
LIF_HUMAN |
| Specificity: |
This kit recognizes Human LIF in samples. No significant cross-reactivity or interference between Human LIF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. |
| Protein Name | Leukemia Inhibitory FactorLifDifferentiation-Stimulating FactorD FactorMelanoma-Derived Lpl InhibitorMlpliEmfilermin |
| Database Links | Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-6788467 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Leukemia Inhibitory Factor CLIA kitLif CLIA kitDifferentiation-Stimulating Factor CLIA kitD Factor CLIA kitMelanoma-Derived Lpl Inhibitor CLIA kitMlpli CLIA kitEmfilermin CLIA kitLIF CLIA kitHILDA CLIA kit |
| Specificity | This kit recognizes Human LIF in samples. No significant cross-reactivity or interference between Human LIF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-108 | 102 |
| EDTA plasma(n=8) | 88-102 | 95 |
| Cell culture media(n=8) | 91-102 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 37.17 | 85.53 | 303.63 | 37.36 | 79.49 | 320.35 |
| Standard deviation | 4.53 | 9.03 | 25.72 | 4.85 | 5.91 | 27.97 |
| CV (%) | 12.19 | 10.56 | 8.47 | 12.98 | 7.43 | 8.73 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
