| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human LGALS13 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
LGALS13 |
| Gene ID: |
29124 |
| Uniprot ID: |
PP13_HUMAN |
| Specificity: |
This kit recognizes Human PP13 in samples. No significant cross-reactivity or interference between Human PP13 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in adult and fetal spleen, fetal kidney, adult urinary bladder and placenta. Placental expression originates predominantly from the syncytiotrophoblast. |
| Function | Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis. Has hemagglutinating activity towards chicken erythrocytes. |
| Protein Name | Galactoside-Binding Soluble Lectin 13Galectin-13Gal-13Placental Tissue Protein 13Pp13Placental Protein 13 |
| Cellular Localisation | CytoplasmNucleus Matrix |
| Alternative CLIA Names | Galactoside-Binding Soluble Lectin 13 CLIA kitGalectin-13 CLIA kitGal-13 CLIA kitPlacental Tissue Protein 13 CLIA kitPp13 CLIA kitPlacental Protein 13 CLIA kitLGALS13 CLIA kitPLAC8 CLIA kit |
| Specificity | This kit recognizes Human PP13 in samples. No significant cross-reactivity or interference between Human PP13 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-101 | 95 |
| EDTA plasma(n=8) | 91-103 | 96 |
| Cell culture media(n=8) | 96-111 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 23.79 | 44.86 | 171.48 | 25.99 | 49.14 | 163.05 |
| Standard deviation | 2.00 | 3.85 | 15.72 | 2.47 | 3.52 | 16.09 |
| CV (%) | 8.41 | 8.58 | 9.17 | 9.50 | 7.16 | 9.87 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
