| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This human LCN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
LCN1 |
| Gene ID: |
3933 |
| Uniprot ID: |
LCN1_HUMAN |
| Specificity: |
This kit recognizes Human LCN1 in samples. No significant cross-reactivity or interference between Human LCN1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Mainly expressed in lachrymal and salivary glands. Also expressed in the prostate. |
| Function | Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket. |
| Protein Name | Lipocalin-1Tear LipocalinTlcTear PrealbuminTpVon Ebner Gland ProteinVeg Protein |
| Database Links | Reactome: R-HSA-804914 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Lipocalin-1 CLIA kitTear Lipocalin CLIA kitTlc CLIA kitTear Prealbumin CLIA kitTp CLIA kitVon Ebner Gland Protein CLIA kitVeg Protein CLIA kitLCN1 CLIA kitVEGP CLIA kit |
| Specificity | This kit recognizes Human LCN1 in samples. No significant cross-reactivity or interference between Human LCN1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-100 | 94 |
| EDTA plasma(n=8) | 88-101 | 94 |
| Cell culture media(n=8) | 101-116 | 108 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.54 | 1.56 | 4.80 | 0.50 | 1.60 | 4.83 |
| Standard deviation | 0.06 | 0.11 | 0.35 | 0.06 | 0.19 | 0.39 |
| CV (%) | 11.11 | 7.05 | 7.29 | 12.00 | 11.88 | 8.07 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
