| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human KRT13 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
KRT13 |
| Gene ID: |
3860 |
| Uniprot ID: |
K1C13_HUMAN |
| Specificity: |
This kit recognizes Human CK-13/KRT13 in samples. No significant cross-reactivity or interference between Human CK-13/KRT13 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. |
| Post Translational Modifications | O-glycosylated.glycans consist of single N-acetylglucosamine residues. |
| Function | Type 1 keratin (Probable). Maintains postnatal tongue mucosal cell homeostasis and tissue organization in response to mechanical stress, potentially via regulation of the G1/S phase cyclins CCNE1 and CCNE2. |
| Protein Name | Keratin - Type I Cytoskeletal 13Cytokeratin-13Ck-13Keratin-13K13 |
| Database Links | Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
| Alternative CLIA Names | Keratin - Type I Cytoskeletal 13 CLIA kitCytokeratin-13 CLIA kitCk-13 CLIA kitKeratin-13 CLIA kitK13 CLIA kitKRT13 CLIA kit |
| Specificity | This kit recognizes Human CK-13/KRT13 in samples. No significant cross-reactivity or interference between Human CK-13/KRT13 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-104 | 96 |
| EDTA plasma(n=8) | 85-98 | 92 |
| Cell culture media(n=8) | 101-117 | 107 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 24.82 | 71.11 | 226.06 | 26.02 | 74.00 | 214.94 |
| Standard deviation | 3.17 | 6.09 | 17.27 | 2.45 | 6.18 | 23.30 |
| CV (%) | 12.77 | 8.56 | 7.64 | 9.42 | 8.35 | 10.84 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
