| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38ng/mL |
| Detection Limit: |
15.63~1000ng/mL |
| Short Description: |
This KRT10 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
KRT10 |
| Gene ID: |
3858 |
| Uniprot ID: |
K1C10_HUMAN |
| Specificity: |
This kit recognizes Human KRT10 in samples. No significant cross-reactivity or interference between Human KRT10 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Plays a role in the establishment of the epidermal barrier on plantar skin. Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium. (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur. |
| Protein Name | Keratin - Type I Cytoskeletal 10Cytokeratin-10Ck-10Keratin-10K10 |
| Database Links | Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
| Cellular Localisation | SecretedExtracellular SpaceCell SurfaceCytoplasm |
| Alternative ELISA Names | Keratin - Type I Cytoskeletal 10 ELISA kitCytokeratin-10 ELISA kitCk-10 ELISA kitKeratin-10 ELISA kitK10 ELISA kitKRT10 ELISA kitKPP ELISA kit |
| Specificity | This kit recognizes Human KRT10 in samples. No significant cross-reactivity or interference between Human KRT10 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-95 | 92 |
| EDTA plasma (n=8) | 83-97 | 90 |
| Cell culture media (n=8) | 100-107 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 47.59 | 106.12 | 433.09 | 46.70 | 103.75 | 435.89 |
| Standard deviation | 2.70 | 5.31 | 21.65 | 2.65 | 4.83 | 19.27 |
| CV (%) | 5.67 | 5.00 | 5.00 | 5.67 | 4.66 | 4.42 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
