| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This KLK7 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
KLK7 |
| Gene ID: |
5650 |
| Uniprot ID: |
KLK7_HUMAN |
| Specificity: |
This kit recognizes Human KLK7 in samples. No significant cross-reactivity or interference between Human KLK7 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines. |
| Protein Name | Kallikrein-7Hk7Serine Protease 6Stratum Corneum Chymotryptic EnzymeHscce |
| Database Links | Reactome: R-HSA-1474228 |
| Cellular Localisation | SecretedIn Ovarian CarcinomaSecreted And Also Observed At The Apical Membrane And In Cytoplasm At The Invasive Front |
| Alternative ELISA Names | Kallikrein-7 ELISA kitHk7 ELISA kitSerine Protease 6 ELISA kitStratum Corneum Chymotryptic Enzyme ELISA kitHscce ELISA kitKLK7 ELISA kitPRSS6 ELISA kitSCCE ELISA kit |
| Specificity | This kit recognizes Human KLK7 in samples. No significant cross-reactivity or interference between Human KLK7 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-104 | 97 |
| EDTA plasma (n=8) | 90-102 | 95 |
| Cell culture media (n=8) | 85-97 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.46 | 1.38 | 3.62 | 0.44 | 1.38 | 3.58 |
| Standard deviation | 0.03 | 0.07 | 0.11 | 0.02 | 0.07 | 0.15 |
| CV (%) | 6.52 | 5.07 | 3.04 | 4.55 | 5.07 | 4.19 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
