| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This IL4I1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
IL4I1 |
| Gene ID: |
259307 |
| Uniprot ID: |
OXLA_HUMAN |
| Specificity: |
This kit recognizes Human IL4I1 in samples. No significant cross-reactivity or interference between Human IL4I1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated. |
| Function | Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively. Also has weak L-arginine oxidase activity. Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR. IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer. AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity. Also has an immunoregulatory function in some immune cells, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells. Also regulates M2 macrophage polarization by inhibiting T-cell activation. Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2. |
| Protein Name | L-Amino-Acid OxidaseLaaoLaoInterleukin-4-Induced Protein 1Il4-Induced Protein 1Hil4i1Protein Fig-1Hfig1 |
| Database Links | Reactome: R-HSA-8964208 |
| Cellular Localisation | SecretedLysosomeCytoplasmic VesicleSecretory VesicleAcrosomeSecreted At The Immunological Synapse |
| Alternative ELISA Names | L-Amino-Acid Oxidase ELISA kitLaao ELISA kitLao ELISA kitInterleukin-4-Induced Protein 1 ELISA kitIl4-Induced Protein 1 ELISA kitHil4i1 ELISA kitProtein Fig-1 ELISA kitHfig1 ELISA kitIL4I1 ELISA kitFIG1 ELISA kitUNQ636 ELISA kitPRO1265 ELISA kit |
| Specificity | This kit recognizes Human IL4I1 in samples. No significant cross-reactivity or interference between Human IL4I1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-99 | 91 |
| EDTA plasma (n=8) | 89-102 | 96 |
| Cell culture media (n=8) | 86-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 1.56 | 4.00 | 0.52 | 1.47 | 4.19 |
| Standard deviation | 0.03 | 0.07 | 0.15 | 0.03 | 0.06 | 0.20 |
| CV (%) | 6.00 | 4.49 | 3.75 | 5.77 | 4.08 | 4.77 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
