| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
56.25pg/mL |
| Detection Limit: |
93.75~6000pg/mL |
| Short Description: |
This IL2RA Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
IL2RA |
| Gene ID: |
3559 |
| Uniprot ID: |
IL2RA_HUMAN |
| Specificity: |
This kit recognizes Human IL-2R Alpha in samples. No significant cross-reactivity or interference between Human IL-2R Alpha and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Protein Name | Interleukin-2 Receptor Subunit AlphaIl-2 Receptor Subunit AlphaIl-2-RaIl-2r Subunit AlphaIl2-RaTac AntigenP55Cd Antigen Cd25 |
| Database Links | Reactome: R-HSA-5673001Reactome: R-HSA-8877330Reactome: R-HSA-9020558Reactome: R-HSA-912526 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative ELISA Names | Interleukin-2 Receptor Subunit Alpha ELISA kitIl-2 Receptor Subunit Alpha ELISA kitIl-2-Ra ELISA kitIl-2r Subunit Alpha ELISA kitIl2-Ra ELISA kitTac Antigen ELISA kitP55 ELISA kitCd Antigen Cd25 ELISA kitIL2RA ELISA kit |
| Specificity | This kit recognizes Human IL-2Rα in samples. No significant cross-reactivity or interference between Human IL-2Rα and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-103 | 94 |
| EDTA plasma (n=8) | 84-100 | 91 |
| Cell culture media (n=8) | 91-106 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 295.49 | 623.35 | 2430.39 | 307.44 | 595.04 | 2280.42 |
| Standard deviation | 16.75 | 30.05 | 122.25 | 19.83 | 23.92 | 85.06 |
| CV (%) | 5.67 | 4.82 | 5.03 | 6.45 | 4.02 | 3.73 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
