| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
13.8pg/mL |
| Detection Limit: |
31.2-2000pg/mL |
| Short Description: |
This IL10Ra Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
IL10RA |
| Gene ID: |
3587 |
| Uniprot ID: |
I10R1_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells. |
| Post Translational Modifications | Phosphorylated. Phosphorylation of the cytoplasmic tail induced STAT3 activation. Ubiquitinated by BTRC.ubiquitination leads to endocytosis and subsequent degradation of IL10RA. |
| Function | Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy. |
| Protein Name | Interleukin-10 Receptor Subunit AlphaIl-10 Receptor Subunit AlphaIl-10r Subunit AlphaIl-10raCdw210aInterleukin-10 Receptor Subunit 1Il-10r Subunit 1Il-10r1Cd Antigen Cd210 |
| Database Links | Reactome: R-HSA-6783783 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasm |
| Alternative ELISA Names | Interleukin-10 Receptor Subunit Alpha ELISA kitIl-10 Receptor Subunit Alpha ELISA kitIl-10r Subunit Alpha ELISA kitIl-10ra ELISA kitCdw210a ELISA kitInterleukin-10 Receptor Subunit 1 ELISA kitIl-10r Subunit 1 ELISA kitIl-10r1 ELISA kitCd Antigen Cd210 ELISA kitIL10RA ELISA kitIL10R ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
