• Human IGF2R (Cation-independent mannose-6-phosphate receptor) CLIA Kit (STJC0000257)

Human IGF2R (Cation-independent mannose-6-phosphate receptor) CLIA Kit (STJC0000257)

SKU:
STJC0000257

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 75pg/mL
Detection Limit: 125~8000pg/mL
Short Description: This human IGF2R kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: IGF2R
Gene ID: 3482
Uniprot ID: MPRI_HUMAN
Specificity: This kit recognizes Human IGF2R in samples. No significant cross-reactivity or interference between Human IGF2R and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Palmitoylated. Undergoes cysteine S-palmitoylation which promotes interaction with the retromer cargo-selective complex which mediates its retrograde trafficking to the Golgi apparatus.
Function Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation by binding DPP4.
Protein Name Cation-Independent Mannose-6-Phosphate Receptor
Ci Man-6-P Receptor
Ci-Mpr
M6pr
300 Kda Mannose 6-Phosphate Receptor
Mpr 300
Insulin-Like Growth Factor 2 Receptor
Insulin-Like Growth Factor Ii Receptor
Igf-Ii Receptor
M6p/Igf2 Receptor
M6p/Igf2r
Cd Antigen Cd222
Database Links Reactome: R-HSA-432722
Reactome: R-HSA-6798695
Reactome: R-HSA-6811440
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type I Membrane Protein
Endosome Membrane
Mainly Localized In The Golgi At Steady State And Not Detectable In Lysosome
Colocalized With Dpp4 In Internalized Cytoplasmic Vesicles Adjacent To The Cell Surface
Alternative CLIA Names Cation-Independent Mannose-6-Phosphate Receptor CLIA kit
Ci Man-6-P Receptor CLIA kit
Ci-Mpr CLIA kit
M6pr CLIA kit
300 Kda Mannose 6-Phosphate Receptor CLIA kit
Mpr 300 CLIA kit
Insulin-Like Growth Factor 2 Receptor CLIA kit
Insulin-Like Growth Factor Ii Receptor CLIA kit
Igf-Ii Receptor CLIA kit
M6p/Igf2 Receptor CLIA kit
M6p/Igf2r CLIA kit
Cd Antigen Cd222 CLIA kit
IGF2R CLIA kit
MPRI CLIA kit
Specificity This kit recognizes Human IGF2R in samples. No significant cross-reactivity or interference between Human IGF2R and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-108 101
EDTA plasma(n=8) 94-106 99
Cell culture media(n=8) 89-104 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 419.94 789.36 3128.14 412.07 809.34 3137.23
Standard deviation 43.38 94.72 253.07 43.47 82.80 209.88
CV (%) 10.33 12.00 8.09 10.55 10.23 6.69
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance