| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.15 ng/mL |
| Detection Limit: |
0312-20 ng/mL |
| Short Description: |
This IGF1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Conjugation: |
Biotin |
| Storage Instruction: |
Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month. |
| Assay Time: |
4 hours |
| Detection: |
Colorimetric |
| Gene Symbol: |
IGF1 |
| Gene ID: |
3479 |
| Uniprot ID: |
IGF1_HUMAN |
| Sample Type: |
serum, plasma and cell culture supernates |
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of 1-14C-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1. |
| Protein Name | Insulin-Like Growth Factor IIgf-IMechano Growth FactorMgfSomatomedin-C |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-2404192Reactome: R-HSA-2428928Reactome: R-HSA-2428933Reactome: R-HSA-381426Reactome: R-HSA-422085 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Insulin-Like Growth Factor I ELISA kitIgf-I ELISA kitMechano Growth Factor ELISA kitMgf ELISA kitSomatomedin-C ELISA kitIGF1 ELISA kitIBP1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
