Human IGF-I protein (Active Recombinant) (C-hFc&His) (STJP001094)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human IGF-I Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence ( Gly 49-Ala 118) of human IGF1 (Accession #NP_001104755.1) fused with an Fc, 6×His tag at the C-terminus.
General Information
| Short Description | Active Recombinant-Human IGF-I-C-hFc&His protein was developed in hek293 cells using the region Gly 49-Ala 118. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its ability to stimulate p70 S6 Kinase (Thr389) and p85 S6 Kinase (Thr412) autophosphorylation in 293T human embryonic kidney cells. 0.01-1 ng/mL of Recombinant Human IGF1 can effectively enhance p70 S6 Kinase (Thr389) and p85 S6 Kinase |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IGF1 |
| Gene ID | 3479 |
| Uniprot ID | IGF1_HUMAN |
| Immunogen | Recombinant Human IGF-I Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence ( Gly 49-Ala 118) of human IGF1 (Accession #NP_001104755.1) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Region | Gly 49-Ala 118 |
| Immunogen Sequence | GPETLCGAELVDALQFVCGD RGFYFNKPTGYGSSSRRAPQ TGIVDECCFRSCDLRRLEMY CAPLKPAKSA |
Additional Info
| Function | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of 1-14C-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM. |
| Protein Name | Insulin-Like Growth Factor 1Insulin-Like Growth Factor IIgf-IMechano Growth FactorMgfSomatomedin-C |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-2404192Reactome: R-HSA-2428928Reactome: R-HSA-2428933Reactome: R-HSA-381426Reactome: R-HSA-422085 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Insulin-Like Growth Factor 1 proteinInsulin-Like Growth Factor I proteinIgf-I proteinMechano Growth Factor proteinMgf proteinSomatomedin-C proteinIGF1 proteinIBP1 proteinIGF-1 protein |
Information sourced from Uniprot.org