| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human HSF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
HSF1 |
| Gene ID: |
3297 |
| Uniprot ID: |
HSF1_HUMAN |
| Specificity: |
This kit recognizes Human HSF1 in samples. No significant cross-reactivity or interference between Human HSF1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated. Phosphorylated in unstressed cells.this phosphorylation is constitutive and implicated in the repression of HSF1 transcriptional activity. Phosphorylated on Ser-121 by MAPKAPK2.this phosphorylation promotes interaction with HSP90 proteins and inhibits HSF1 homotrimerization, DNA-binding and transactivation activities. Phosphorylation on Ser-303 by GSK3B/GSK3-beta and on Ser-307 by MAPK3 within the regulatory domain is involved in the repression of HSF1 transcriptional activity and occurs in a RAF1-dependent manner. Phosphorylation on Ser-303 and Ser-307 increases HSF1 nuclear export in a YWHAE- and XPO1/CRM1-dependent manner. Phosphorylation on Ser-307 is a prerequisite for phosphorylation on Ser-303. Ser-303 is not phosphorylated in unstressed cells. Phosphorylated on Ser-419 by PLK1.phosphorylation promotes nuclear translocation upon heat shock. Hyperphosphorylated upon heat shock and during the attenuation and recovery phase period of the heat shock response. Phosphorylated on Thr-142.this phosphorylation increases HSF1 transactivation activity upon heat shock. Phosphorylation on Ser-230 by CAMK2A.this phosphorylation enhances HSF1 transactivation activity upon heat shock. Phosphorylation on Ser-326 by MAPK12.this phosphorylation enhances HSF1 nuclear translocation, homotrimerization and transactivation activities upon heat shock. Phosphorylated on Ser-320 by PRKACA/PKA.this phosphorylation promotes nuclear localization and transcriptional activity upon heat shock. Phosphorylated on Ser-363 by MAPK8.this phosphorylation occurs upon heat shock, induces HSF1 translocation into nuclear stress bodies and negatively regulates transactivation activity. Neither basal nor stress-inducible phosphorylation on Ser-230, Ser-292, Ser-303, Ser-307, Ser-314, Ser-319, Ser-320, Thr-323, Ser-326, Ser-338, Ser-344, Ser-363, Thr-367, Ser-368 and Thr-369 within the regulatory domain is involved in the regulation of HSF1 subcellular localization or DNA-binding activity.however, it negatively regulates HSF1 transactivation activity. Phosphorylated on Ser-216 by PLK1 in the early mitotic period.this phosphorylation regulates HSF1 localization to the spindle pole, the recruitment of the SCF(BTRC) ubiquitin ligase complex inducing HSF1 degradation, and hence mitotic progression. Dephosphorylated on Ser-121, Ser-307, Ser-314, Thr-323 and Thr-367 by phosphatase PPP2CA in an IER5-dependent manner, leading to HSF1-mediated transactivation activity. Sumoylated with SUMO1 and SUMO2 upon heat shock in a ERK2-dependent manner. Sumoylated by SUMO1 on Lys-298.sumoylation occurs upon heat shock and promotes its localization to nuclear stress bodies and DNA-binding activity. Phosphorylation on Ser-303 and Ser-307 is probably a prerequisite for sumoylation. Acetylated on Lys-118.this acetylation is decreased in a IER5-dependent manner. Acetylated on Lys-118, Lys-208 and Lys-298.these acetylations occur in a EP300-dependent manner. Acetylated on Lys-80.this acetylation inhibits DNA-binding activity upon heat shock. Deacetylated on Lys-80 by SIRT1.this deacetylation increases DNA-binding activity. Ubiquitinated by SCF(BTRC) and degraded following stimulus-dependent phosphorylation at Ser-216 by PLK1 in mitosis. Polyubiquitinated. Undergoes proteasomal degradation upon heat shock and during the attenuation and recovery phase period of the heat shock response. |
| Function | Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones, heat shock proteins (HSPs), that protect cells from cellular insult damage. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes. Upon heat shock stress, forms a chromatin-associated complex with TTC5/STRAP and p300/EP300 to stimulate HSR transcription, therefore increasing cell survival. Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form. Binds to inverted 5'-NGAAN-3' pentamer DNA sequences. Binds to chromatin at heat shock gene promoters. Activates transcription of transcription factor FOXR1 which in turn activates transcription of the heat shock chaperones HSPA1A and HSPA6 and the antioxidant NADPH-dependent reductase DHRS2. Also serves several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells. Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner. Plays a role in nuclear export of stress-induced HSP70 mRNA. Plays a role in the regulation of mitotic progression. Also plays a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner. Involved in stress-induced cancer cell proliferation in a IER5-dependent manner. (Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300. |
| Protein Name | Heat Shock Factor Protein 1Hsf 1Heat Shock Transcription Factor 1Hstf 1 |
| Database Links | Reactome: R-HSA-3371453Reactome: R-HSA-3371511Reactome: R-HSA-3371568Reactome: R-HSA-3371571Reactome: R-HSA-9646399 |
| Cellular Localisation | NucleusCytoplasmNucleoplasmPerinuclear RegionCytoskeletonSpindle PoleMicrotubule Organizing CenterCentrosomeChromosomeCentromereKinetochoreThe Monomeric Form Is Cytoplasmic In Unstressed CellsPredominantly Nuclear Protein In Both Unstressed And Heat Shocked CellsTranslocates In The Nucleus Upon Heat ShockNucleocytoplasmic Shuttling ProteinColocalizes With Ier5 In The NucleusColocalizes With Bag3 To The Nucleus Upon Heat StressLocalizes In Subnuclear Granules Called Nuclear Stress Bodies (Nsbs) Upon Heat ShockColocalizes With Sympk And Sumo1 In Nsbs Upon Heat ShockColocalizes With Prkaca/Pka In The Nucleus And Nsbs Upon Heat ShockRelocalizes From The Nucleus To The Cytoplasm During The Attenuation And Recovery Phase Period Of The Heat Shock ResponseTranslocates In The Cytoplasm In A Ywhae- And Xpo1/Crm1-Dependent MannerTogether With Histone H2axRedistributed In Discrete Nuclear Dna Damage-Induced Foci After Ionizing Radiation (Ir)Colocalizes With Calcium-Responsive Transactivator Ss18l1 At Kinetochore Region On The Mitotic ChromosomesColocalizes With Gamma Tubulin At CentrosomeLocalizes At Spindle Pole In MetaphaseColocalizes With Plk1 At Spindle Poles During Prometaphase |
| Alternative CLIA Names | Heat Shock Factor Protein 1 CLIA kitHsf 1 CLIA kitHeat Shock Transcription Factor 1 CLIA kitHstf 1 CLIA kitHSF1 CLIA kitHSTF1 CLIA kit |
| Specificity | This kit recognizes Human HSF1 in samples. No significant cross-reactivity or interference between Human HSF1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-101 | 93 |
| EDTA plasma(n=8) | 94-108 | 102 |
| Cell culture media(n=8) | 98-111 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 201.95 | 621.84 | 1686.34 | 199.52 | 625.53 | 1839.13 |
| Standard deviation | 16.46 | 67.41 | 128.16 | 24.86 | 56.80 | 111.45 |
| CV (%) | 8.15 | 10.84 | 7.60 | 12.46 | 9.08 | 6.06 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
