| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.057ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This HSD11b2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
HSD11B2 |
| Gene ID: |
3291 |
| Uniprot ID: |
DHI2_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in kidney, placenta, pancreas, prostate, ovary, small intestine and colon, and in lower levels in the spleen and testis. At midgestation, expressed at high levels in placenta and in fetal kidney and, at much lower levels, in fetal lung and testis. |
| Post Translational Modifications | |
| Function | Catalyzes the conversion of biologically active 11beta-hydroxyglucocorticoids (11beta-hydroxysteroid) such as cortisol, to inactive 11-ketoglucocorticoids (11-oxosteroid) such as cortisone, in the presence of NAD(+). Functions as a dehydrogenase (oxidase), thereby decreasing the concentration of active glucocorticoids, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Plays an important role in maintaining glucocorticoids balance during preimplantation and protects the fetus from excessive maternal corticosterone exposure. Catalyzes the oxidation of 11beta-hydroxytestosterone (11beta,17beta-dihydroxyandrost-4-ene-3-one) to 11-ketotestosterone (17beta-hydroxyandrost-4-ene-3,11-dione), a major bioactive androgen. Catalyzes the conversion of 11beta-hydroxyandrostenedione (11beta-hydroxyandrost-4-ene-3,17-dione) to 11-ketoandrostenedione (androst-4-ene-3,11,17-trione), which can be further metabolized to 11-ketotestosterone. Converts 7-beta-25-dihydroxycholesterol to 7-oxo-25-hydroxycholesterol in vitro. 7-beta-25-dihydroxycholesterol (not 7-oxo-25-hydroxycholesterol) acts as ligand for the G-protein-coupled receptor (GPCR) Epstein-Barr virus-induced gene 2 (EBI2) and may thereby regulate immune cell migration. May protect ovulating oocytes and fertilizing spermatozoa from the adverse effects of cortisol. |
| Protein Name | 11-Beta-Hydroxysteroid Dehydrogenase Type 211-Dh211-Beta-Hsd211-Beta-Hydroxysteroid Dehydrogenase Type Ii11-Hsd Type Ii11-Beta-Hsd Type IiCorticosteroid 11-Beta-Dehydrogenase Isozyme 2Nad-Dependent 11-Beta-Hydroxysteroid DehydrogenaseShort Chain Dehydrogenase/Reductase Family 9c Member 3 |
| Database Links | Reactome: R-HSA-194002Reactome: R-HSA-9757110 |
| Cellular Localisation | MicrosomeEndoplasmic Reticulum |
| Alternative ELISA Names | 11-Beta-Hydroxysteroid Dehydrogenase Type 2 ELISA kit11-Dh2 ELISA kit11-Beta-Hsd2 ELISA kit11-Beta-Hydroxysteroid Dehydrogenase Type Ii ELISA kit11-Hsd Type Ii ELISA kit11-Beta-Hsd Type Ii ELISA kitCorticosteroid 11-Beta-Dehydrogenase Isozyme 2 ELISA kitNad-Dependent 11-Beta-Hydroxysteroid Dehydrogenase ELISA kitShort Chain Dehydrogenase/Reductase Family 9c Member 3 ELISA kitHSD11B2 ELISA kitHSD11K ELISA kitSDR9C3 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
