• Human GT-34 (Gastrin 34) CLIA Kit (STJC0000869)

Human GT-34 (Gastrin 34) CLIA Kit (STJC0000869)

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STJC0000869

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This human GT-34 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: GAST
Gene ID: 2520
Uniprot ID: GAST_HUMAN
Specificity: This kit recognizes Human GT-34 in samples. No significant cross-reactivity or interference between Human GT-34 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Two different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71. Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins. Thus, gastrin-6 is almost 73% sulfated, whereas the larger gastrins are less than 50% sulfated. Sulfation levels are also tissue-specific.
Function Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Protein Name Gastrin Cleaved Into - Gastrin-71
Gastrin Component I - Gastrin-52
G52 - Big Gastrin
Gastrin Component Ii
Gastrin-34
G34 - Gastrin
Gastrin Component Iii
Gastrin-17
G17 - Gastrin-14
G14 - Gastrin-6
G6
Database Links Reactome: R-HSA-416476
Reactome: R-HSA-881907
Cellular Localisation Secreted
Alternative CLIA Names Gastrin Cleaved Into - Gastrin-71 CLIA kit
Gastrin Component I - Gastrin-52 CLIA kit
G52 - Big Gastrin CLIA kit
Gastrin Component Ii CLIA kit
Gastrin-34 CLIA kit
G34 - Gastrin CLIA kit
Gastrin Component Iii CLIA kit
Gastrin-17 CLIA kit
G17 - Gastrin-14 CLIA kit
G14 - Gastrin-6 CLIA kit
G6 CLIA kit
GAST CLIA kit
GAS CLIA kit
Specificity This kit recognizes Human GT-34 in samples. No significant cross-reactivity or interference between Human GT-34 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-107 100
EDTA plasma(n=8) 91-105 98
Cell culture media(n=8) 94-108 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 24.37 60.04 228.40 24.87 64.70 249.03
Standard deviation 2.78 6.04 25.70 2.75 6.13 28.36
CV (%) 11.41 10.06 11.25 11.06 9.47 11.39
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance