| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human GT-34 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
GAST |
| Gene ID: |
2520 |
| Uniprot ID: |
GAST_HUMAN |
| Specificity: |
This kit recognizes Human GT-34 in samples. No significant cross-reactivity or interference between Human GT-34 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Two different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71. Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins. Thus, gastrin-6 is almost 73% sulfated, whereas the larger gastrins are less than 50% sulfated. Sulfation levels are also tissue-specific. |
| Function | Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. |
| Protein Name | Gastrin Cleaved Into - Gastrin-71Gastrin Component I - Gastrin-52G52 - Big GastrinGastrin Component IiGastrin-34G34 - GastrinGastrin Component IiiGastrin-17G17 - Gastrin-14G14 - Gastrin-6G6 |
| Database Links | Reactome: R-HSA-416476Reactome: R-HSA-881907 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Gastrin Cleaved Into - Gastrin-71 CLIA kitGastrin Component I - Gastrin-52 CLIA kitG52 - Big Gastrin CLIA kitGastrin Component Ii CLIA kitGastrin-34 CLIA kitG34 - Gastrin CLIA kitGastrin Component Iii CLIA kitGastrin-17 CLIA kitG17 - Gastrin-14 CLIA kitG14 - Gastrin-6 CLIA kitG6 CLIA kitGAST CLIA kitGAS CLIA kit |
| Specificity | This kit recognizes Human GT-34 in samples. No significant cross-reactivity or interference between Human GT-34 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-107 | 100 |
| EDTA plasma(n=8) | 91-105 | 98 |
| Cell culture media(n=8) | 94-108 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 24.37 | 60.04 | 228.40 | 24.87 | 64.70 | 249.03 |
| Standard deviation | 2.78 | 6.04 | 25.70 | 2.75 | 6.13 | 28.36 |
| CV (%) | 11.41 | 10.06 | 11.25 | 11.06 | 9.47 | 11.39 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
