Applications: |
ELISA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
0.19ng/mL |
Detection Limit: |
0.31~20ng/mL |
Short Description: |
This GSTA1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Colormetric |
Gene Symbol: |
GSTA1 |
Gene ID: |
2938 |
Uniprot ID: |
GSTA1_HUMAN |
Specificity: |
This kit recognizes Human GST Alpha 1 in samples. No significant cross-reactivity or interference between Human GST Alpha 1 and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Function | Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid. |
Protein Name | Glutathione S-Transferase A113-Hydroperoxyoctadecadienoate PeroxidaseAndrost-5-Ene-3 -17-Dione IsomeraseGst Ha Subunit 1Gst Class-Alpha Member 1Gst-EpsilonGsta1-1Gth1 Cleaved Into - Glutathione S-Transferase A1 - N-Terminally Processed |
Database Links | Reactome: R-HSA-156590Reactome: R-HSA-189483Reactome: R-HSA-9748787Reactome: R-HSA-9818027 |
Cellular Localisation | Cytoplasm |
Alternative ELISA Names | Glutathione S-Transferase A1 ELISA kit13-Hydroperoxyoctadecadienoate Peroxidase ELISA kitAndrost-5-Ene-3 -17-Dione Isomerase ELISA kitGst Ha Subunit 1 ELISA kitGst Class-Alpha Member 1 ELISA kitGst-Epsilon ELISA kitGsta1-1 ELISA kitGth1 Cleaved Into - Glutathione S-Transferase A1 - N-Terminally Processed ELISA kitGSTA1 ELISA kit |
Specificity | This kit recognizes Human GSTα1 in samples. No significant cross-reactivity or interference between Human GSTα1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 100-111 | 103 |
EDTA plasma (n=8) | 89-101 | 94 |
Cell culture media (n=8) | 101-111 | 104 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 0.86 | 2.28 | 8.66 | 0.90 | 2.00 | 8.79 |
Standard deviation | 0.05 | 0.11 | 0.44 | 0.05 | 0.09 | 0.35 |
CV (%) | 5.81 | 4.82 | 5.08 | 5.56 | 4.50 | 3.98 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance