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Human GRB2 (Growth factor receptor-bound protein 2) CLIA Kit (STJC0000840)

Human GRB2 (Growth factor receptor-bound protein 2) CLIA Kit (STJC0000840)

Human GRB2 (Growth factor receptor-bound protein 2) CLIA Kit (STJC0000840)

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STJC0000840

€298.13 - €596.88

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	18.75pg/mL
Detection Limit:	31.25~2000pg/mL
Short Description:	This human GRB2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	GRB2
Gene ID:	2885
Uniprot ID:	GRB2_HUMAN
Specificity:	This kit recognizes Human Grb2 in samples. No significant cross-reactivity or interference between Human Grb2 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Isoform 2: Does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Protein Name	Growth Factor Receptor-Bound Protein 2 Adapter Protein Grb2 Protein Ash Sh2/Sh3 Adapter Grb2
Database Links	Reactome: R-HSA-109704 P62993-1 Reactome: R-HSA-112412 P62993-1 Reactome: R-HSA-1236382 P62993-1 Reactome: R-HSA-1250196 P62993-1 Reactome: R-HSA-1250347 P62993-1 Reactome: R-HSA-1257604 P62993-1 Reactome: R-HSA-1295596 P62993-1 Reactome: R-HSA-1433557 P62993-1 Reactome: R-HSA-1433559 P62993-1 Reactome: R-HSA-167044 P62993-1 Reactome: R-HSA-179812 P62993-1 Reactome: R-HSA-180292 P62993-1 Reactome: R-HSA-180336 P62993-1 Reactome: R-HSA-182971 P62993-1 Reactome: R-HSA-1839117 P62993-1 Reactome: R-HSA-186763 P62993-1 Reactome: R-HSA-1963640 P62993-1 Reactome: R-HSA-1963642 P62993-1 Reactome: R-HSA-2029482 P62993-1 Reactome: R-HSA-210993 P62993-1 Reactome: R-HSA-2179392 P62993-1 Reactome: R-HSA-2219530 P62993-1 Reactome: R-HSA-2424491 P62993-1 Reactome: R-HSA-2428933 P62993-1 Reactome: R-HSA-2730905 P62993-1 Reactome: R-HSA-2871796 P62993-1 Reactome: R-HSA-2871809 P62993-1 Reactome: R-HSA-354194 P62993-1 Reactome: R-HSA-375165 P62993-1 Reactome: R-HSA-388841 P62993-1 Reactome: R-HSA-389359 P62993-1 Reactome: R-HSA-391160 P62993-1 Reactome: R-HSA-512988 P62993-1 Reactome: R-HSA-5637810 P62993-1 Reactome: R-HSA-5654688 P62993-1 Reactome: R-HSA-5654689 P62993-1 Reactome: R-HSA-5654693 P62993-1 Reactome: R-HSA-5654695 P62993-1 Reactome: R-HSA-5654699 P62993-1 Reactome: R-HSA-5654700 P62993-1 Reactome: R-HSA-5654704 P62993-1 Reactome: R-HSA-5654706 P62993-1 Reactome: R-HSA-5654710 P62993-1 Reactome: R-HSA-5654712 P62993-1 Reactome: R-HSA-5654719 P62993-1 Reactome: R-HSA-5654720 P62993-1 Reactome: R-HSA-5654726 P62993-1 Reactome: R-HSA-5654727 P62993-1 Reactome: R-HSA-5654732 P62993-1 Reactome: R-HSA-5654733 P62993-1 Reactome: R-HSA-5655253 P62993-1 Reactome: R-HSA-5655291 P62993-1 Reactome: R-HSA-5655302 P62993-1 Reactome: R-HSA-5655332 P62993-1 Reactome: R-HSA-5663213 P62993-1 Reactome: R-HSA-5673001 P62993-1 Reactome: R-HSA-6807004 P62993-1 Reactome: R-HSA-6811558 P62993-1 Reactome: R-HSA-74749 P62993-1 Reactome: R-HSA-74751 P62993-1 Reactome: R-HSA-8851805 P62993-1 Reactome: R-HSA-8851907 P62993-1 Reactome: R-HSA-8853659 P62993-1 Reactome: R-HSA-8856825 P62993-1 Reactome: R-HSA-8856828 P62993-1 Reactome: R-HSA-8865999 P62993-1 Reactome: R-HSA-8875360 P62993-1 Reactome: R-HSA-8875555 P62993-1 Reactome: R-HSA-8875656 P62993-1 Reactome: R-HSA-8983432 Reactome: R-HSA-9013420 P62993-1 Reactome: R-HSA-9026519 P62993-1 Reactome: R-HSA-9027284 P62993-1 Reactome: R-HSA-9028335 P62993-1 Reactome: R-HSA-9028731 P62993-1 Reactome: R-HSA-9034864 P62993-1 Reactome: R-HSA-912526 P62993-1 Reactome: R-HSA-912631 P62993-1 Reactome: R-HSA-9607240 P62993-1 Reactome: R-HSA-9634285 P62993-1 Reactome: R-HSA-9645135 P62993-1 Reactome: R-HSA-9664422 P62993-1 Reactome: R-HSA-9664565 P62993-1 Reactome: R-HSA-9665348 P62993-1 Reactome: R-HSA-9665686 P62993-1 Reactome: R-HSA-9670439 P62993-1 Reactome: R-HSA-9673767 P62993-1 Reactome: R-HSA-9673770 P62993-1 Reactome: R-HSA-9674555 Reactome: R-HSA-9679191 P62993-1 Reactome: R-HSA-9680350 P62993-1 Reactome: R-HSA-9702518 Reactome: R-HSA-9703465 P62993-1 Reactome: R-HSA-9703648 Reactome: R-HSA-9725370 Reactome: R-HSA-983695 P62993-1
Cellular Localisation	Nucleus Cytoplasm Endosome Golgi Apparatus
Alternative CLIA Names	Growth Factor Receptor-Bound Protein 2 CLIA kit Adapter Protein Grb2 CLIA kit Protein Ash CLIA kit Sh2/Sh3 Adapter Grb2 CLIA kit GRB2 CLIA kit ASH CLIA kit
Specificity	This kit recognizes Human Grb2 in samples. No significant cross-reactivity or interference between Human Grb2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-102	93
EDTA plasma(n=8)	97-110	102
Cell culture media(n=8)	91-104	97

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	95.06	321.08	693.98	95.44	350.05	692.91
Standard deviation	8.57	27.29	76.34	11.53	39.24	46.42
CV (%)	9.02	8.50	11.00	12.08	11.21	6.70

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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