| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This GRB14 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
GRB14 |
| Gene ID: |
2888 |
| Uniprot ID: |
GRB14_HUMAN |
| Specificity: |
This kit recognizes Human Grb14 in samples. No significant cross-reactivity or interference between Human Grb14 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2. |
| Function | Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal. |
| Protein Name | Growth Factor Receptor-Bound Protein 14Grb14 Adapter Protein |
| Database Links | Reactome: R-HSA-210993 |
| Cellular Localisation | CytoplasmEndosome MembranePeripheral Membrane ProteinUpon Insulin StimulationTranslocates To The Plasma Membrane |
| Alternative ELISA Names | Growth Factor Receptor-Bound Protein 14 ELISA kitGrb14 Adapter Protein ELISA kitGRB14 ELISA kit |
| Specificity | This kit recognizes Human Grb14 in samples. No significant cross-reactivity or interference between Human Grb14 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-103 | 97 |
| EDTA plasma (n=8) | 86-103 | 94 |
| Cell culture media (n=8) | 96-110 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.47 | 1.05 | 4.30 | 0.45 | 1.01 | 4.08 |
| Standard deviation | 0.03 | 0.05 | 0.15 | 0.03 | 0.04 | 0.17 |
| CV (%) | 6.38 | 4.76 | 3.49 | 6.67 | 3.96 | 4.17 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
