• Human GRB14 (Growth factor receptor-bound protein 14) Sandwich ELISA (STJE0003330)

Human GRB14 (Growth factor receptor-bound protein 14) Sandwich ELISA (STJE0003330)

SKU:
STJE0003330

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This GRB14 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GRB14
Gene ID: 2888
Uniprot ID: GRB14_HUMAN
Specificity: This kit recognizes Human Grb14 in samples. No significant cross-reactivity or interference between Human Grb14 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2.
Function Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.
Protein Name Growth Factor Receptor-Bound Protein 14
Grb14 Adapter Protein
Database Links Reactome: R-HSA-210993
Cellular Localisation Cytoplasm
Endosome Membrane
Peripheral Membrane Protein
Upon Insulin Stimulation
Translocates To The Plasma Membrane
Alternative ELISA Names Growth Factor Receptor-Bound Protein 14 ELISA kit
Grb14 Adapter Protein ELISA kit
GRB14 ELISA kit
Specificity This kit recognizes Human Grb14 in samples. No significant cross-reactivity or interference between Human Grb14 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 97
EDTA plasma (n=8) 86-103 94
Cell culture media (n=8) 96-110 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.47 1.05 4.30 0.45 1.01 4.08
Standard deviation 0.03 0.05 0.15 0.03 0.04 0.17
CV (%) 6.38 4.76 3.49 6.67 3.96 4.17
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance