| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88ng/mL |
| Detection Limit: |
78.13~5000ng/mL |
| Short Description: |
This GPS1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
GPS1 |
| Gene ID: |
2873 |
| Uniprot ID: |
CSN1_HUMAN |
| Specificity: |
This kit recognizes Human GPS1 in samples. No significant cross-reactivity or interference between Human GPS1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein- and mitogen-activated protein kinase-mediated signal transduction. |
| Protein Name | Cop9 Signalosome Complex Subunit 1Sgn1Signalosome Subunit 1G Protein Pathway Suppressor 1Gps-1Jab1-Containing Signalosome Subunit 1Protein Mfh |
| Database Links | Reactome: R-HSA-5696394Reactome: R-HSA-6781823Reactome: R-HSA-8856825Reactome: R-HSA-8951664Reactome: R-HSA-9013422 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative ELISA Names | Cop9 Signalosome Complex Subunit 1 ELISA kitSgn1 ELISA kitSignalosome Subunit 1 ELISA kitG Protein Pathway Suppressor 1 ELISA kitGps-1 ELISA kitJab1-Containing Signalosome Subunit 1 ELISA kitProtein Mfh ELISA kitGPS1 ELISA kitCOPS1 ELISA kitCSN1 ELISA kit |
| Specificity | This kit recognizes Human GPS1 in samples. No significant cross-reactivity or interference between Human GPS1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-100 | 93 |
| EDTA plasma (n=8) | 90-103 | 96 |
| Cell culture media (n=8) | 89-103 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 269.93 | 606.86 | 2458.91 | 255.51 | 664.56 | 2396.01 |
| Standard deviation | 17.73 | 28.58 | 82.62 | 15.51 | 36.28 | 100.63 |
| CV (%) | 6.57 | 4.71 | 3.36 | 6.07 | 5.46 | 4.20 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
