| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This GPER1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
GPER1 |
| Gene ID: |
2852 |
| Uniprot ID: |
GPER1_HUMAN |
| Specificity: |
This kit recognizes Human GPER1 in samples. No significant cross-reactivity or interference between Human GPER1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Ubiquitinated.ubiquitination occurs at the plasma membrane and leads to proteasome-mediated degradation. Glycosylated. |
| Function | G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK. Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems. Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner. Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation. Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells. Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation. Stimulates uterine epithelial cell proliferation. Enhances uterine contractility in response to oxytocin. Contributes to thymic atrophy by inducing apoptosis. Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules. Promotes neuritogenesis in developing hippocampal neurons. Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death. Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. Inhibits early osteoblast proliferation at growth plate during skeletal development. Inhibits mature adipocyte differentiation and lipid accumulation. Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells. Functions also as a receptor for aldosterone mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway. Involved in cancer progression regulation. Stimulates cancer-associated fibroblast (CAF) proliferation by a rapid genomic response through the EGFR/ERK transduction pathway. Associated with EGFR, may act as a transcription factor activating growth regulatory genes (c-fos, cyclin D1). Promotes integrin alpha-5/beta-1 and fibronectin (FN) matrix assembly in breast cancer cells. |
| Protein Name | G-Protein Coupled Estrogen Receptor 1Chemoattractant Receptor-Like 2Flow-Induced Endothelial G-Protein Coupled Receptor 1Feg-1G Protein-Coupled Estrogen Receptor 1G-Protein Coupled Receptor 30Gpcr-BrIl8-Related Receptor Dry12Lymphocyte-Derived G-Protein Coupled ReceptorLygprMembrane Estrogen ReceptorMer |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-418594Reactome: R-HSA-9634597 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionCytoskeletonCell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneCytoplasmic Vesicle MembraneEarly EndosomeRecycling EndosomeGolgi Apparatus MembraneGolgi ApparatusTrans-Golgi NetworkEndoplasmic Reticulum MembraneCell ProjectionDendriteDendritic Spine MembraneAxonPostsynaptic DensityMitochondrion MembraneColocalized With Bsn To The Active Zone Of Presynaptic DensityColocalized With Dlg4/Psd95 And Neurabin-2 Ppp1r9b In Neuronal SynaptosomesEndocytosed In A Agonist- And Arrestin-Independent MannerColocalized With Ramp3 And Clathrin-Coated Pits At The Plasma MembraneColocalized With Transferrin Receptor At The Plasma Membrane And Perinuclear RegionAccumulated And Colocalized With Rab11 Proteins In Recycling Endosomes And Trans-Golgi Network (Tgn)But Does Neither Recycle Back To The Cell Surface Nor Traffics To Late Endosome Or LysosomeColocalized With Calnexin In The Endoplasmic ReticulumTraffics To Intracellular Sites Via Cytokeratin Intermediate Filaments Like Krt7 And Krt8 After Constitutive Endocytosis In Epithelial CellsColocalized With Egfr In The Nucleus Of Agonist-Induced Cancer-Associated Fibroblasts (Caf) |
| Alternative ELISA Names | G-Protein Coupled Estrogen Receptor 1 ELISA kitChemoattractant Receptor-Like 2 ELISA kitFlow-Induced Endothelial G-Protein Coupled Receptor 1 ELISA kitFeg-1 ELISA kitG Protein-Coupled Estrogen Receptor 1 ELISA kitG-Protein Coupled Receptor 30 ELISA kitGpcr-Br ELISA kitIl8-Related Receptor Dry12 ELISA kitLymphocyte-Derived G-Protein Coupled Receptor ELISA kitLygpr ELISA kitMembrane Estrogen Receptor ELISA kitMer ELISA kitGPER1 ELISA kitCEPR ELISA kitCMKRL2 ELISA kitDRY12 ELISA kitGPER ELISA kitGPR30 ELISA kit |
| Specificity | This kit recognizes Human GPER1 in samples. No significant cross-reactivity or interference between Human GPER1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-104 | 99 |
| EDTA plasma (n=8) | 87-97 | 92 |
| Cell culture media (n=8) | 85-97 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.94 | 2.14 | 7.31 | 0.86 | 2.18 | 7.34 |
| Standard deviation | 0.05 | 0.12 | 0.34 | 0.05 | 0.09 | 0.39 |
| CV (%) | 5.32 | 5.61 | 4.65 | 5.81 | 4.13 | 5.31 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
