Applications: | ELISA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 0.19ng/mL |
Detection Limit: | 0.31~20ng/mL |
Short Description: | This GDNF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | GDNF |
Gene ID: | 2668 |
Uniprot ID: | GDNF_HUMAN |
Specificity: | This kit recognizes Human GDNF in samples. No significant cross-reactivity or interference between Human GDNF and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. |
Protein Name | Glial Cell Line-Derived Neurotrophic Factor Hgdnf Astrocyte-Derived Trophic Factor Atf |
Database Links | Reactome: R-HSA-419037 Reactome: R-HSA-5673001 Reactome: R-HSA-8853659 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Glial Cell Line-Derived Neurotrophic Factor ELISA kit Hgdnf ELISA kit Astrocyte-Derived Trophic Factor ELISA kit Atf ELISA kit GDNF ELISA kit |
Specificity | This kit recognizes Human GDNF in samples. No significant cross-reactivity or interference between Human GDNF and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 92-104 | 98 |
EDTA plasma (n=8) | 87-99 | 92 |
Cell culture media (n=8) | 86-98 | 92 |
Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
---|---|---|---|---|---|---|
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 1.06 | 2.18 | 9.18 | 1.06 | 2.09 | 9.23 |
Standard deviation | 0.06 | 0.11 | 0.35 | 0.07 | 0.11 | 0.40 |
CV (%) | 5.66 | 5.05 | 3.81 | 6.60 | 5.26 | 4.33 |