• Human GDNF (Glial cell line-derived neurotrophic factor) Sandwich ELISA (STJE0003010)

Human GDNF (Glial cell line-derived neurotrophic factor) Sandwich ELISA (STJE0003010)

SKU:
STJE0003010

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This GDNF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GDNF
Gene ID: 2668
Uniprot ID: GDNF_HUMAN
Specificity: This kit recognizes Human GDNF in samples. No significant cross-reactivity or interference between Human GDNF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Protein Name Glial Cell Line-Derived Neurotrophic Factor
Hgdnf
Astrocyte-Derived Trophic Factor
Atf
Database Links Reactome: R-HSA-419037
Reactome: R-HSA-5673001
Reactome: R-HSA-8853659
Cellular Localisation Secreted
Alternative ELISA Names Glial Cell Line-Derived Neurotrophic Factor ELISA kit
Hgdnf ELISA kit
Astrocyte-Derived Trophic Factor ELISA kit
Atf ELISA kit
GDNF ELISA kit
Specificity This kit recognizes Human GDNF in samples. No significant cross-reactivity or interference between Human GDNF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-104 98
EDTA plasma (n=8) 87-99 92
Cell culture media (n=8) 86-98 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.06 2.18 9.18 1.06 2.09 9.23
Standard deviation 0.06 0.11 0.35 0.07 0.11 0.40
CV (%) 5.66 5.05 3.81 6.60 5.26 4.33