Applications: |
ELISA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
46.88pg/mL |
Detection Limit: |
78.13~5000pg/mL |
Short Description: |
This GAD2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Colormetric |
Gene Symbol: |
GAD2 |
Gene ID: |
2572 |
Uniprot ID: |
DCE2_HUMAN |
Specificity: |
This kit recognizes Human GAD2 in samples. No significant cross-reactivity or interference between Human GAD2 and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Function | Catalyzes the production of GABA. |
Protein Name | Glutamate Decarboxylase 265 Kda Glutamic Acid DecarboxylaseGad-65Glutamate Decarboxylase 65 Kda Isoform |
Database Links | Reactome: R-HSA-888568Reactome: R-HSA-888590Reactome: R-HSA-9022927 |
Cellular Localisation | CytoplasmCytosolCytoplasmic VesiclePresynaptic Cell MembraneLipid-AnchorGolgi Apparatus MembranePeripheral Membrane ProteinCytoplasmic SideAssociated To Cytoplasmic VesiclesIn NeuronsCytosolic Leaflet Of Golgi Membranes And Presynaptic Clusters |
Alternative ELISA Names | Glutamate Decarboxylase 2 ELISA kit65 Kda Glutamic Acid Decarboxylase ELISA kitGad-65 ELISA kitGlutamate Decarboxylase 65 Kda Isoform ELISA kitGAD2 ELISA kitGAD65 ELISA kit |
Specificity | This kit recognizes Human GAD2 in samples. No significant cross-reactivity or interference between Human GAD2 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 89-103 | 97 |
EDTA plasma (n=8) | 101-107 | 104 |
Cell culture media (n=8) | 87-95 | 92 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 209.71 | 556.55 | 2120.82 | 232.74 | 603.91 | 2042.33 |
Standard deviation | 11.32 | 26.10 | 104.13 | 12.75 | 28.20 | 88.02 |
CV (%) | 5.40 | 4.69 | 4.91 | 5.48 | 4.67 | 4.31 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance