| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.062ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This FUT3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
FUT3 |
| Gene ID: |
2525 |
| Uniprot ID: |
FUT3_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver. |
| Post Translational Modifications | Glycosylated. |
| Function | Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to both the subterminal N-acetyl glucosamine (GlcNAc) of type 1 chain (beta-D-Gal-(1->3)-beta-D-GlcNAc) glycolipids and oligosaccharides via an alpha(1,4) linkage, and the subterminal glucose (Glc) or GlcNAc of type 2 chain (beta-D-Gal-(1->4)-beta-D-GlcNAc) oligosaccharides via an alpha(1,3) linkage, independently of the presence of terminal alpha-L-fucosyl-(1,2) moieties on the terminal galactose of these acceptors and participates in the blood groups Lewis determination and expression of Lewis a (Le(a)), lewis b (Le(b)), Lewis x/SSEA-1 (Le(x)) and lewis y (Le(y)) antigens. Also catalyzes the transfer of L-fucose to subterminal GlcNAc of sialyl- and disialyl-lactotetraosylceramide to produce sialyl Lewis a (sLe(a)) and disialyl Lewis a via an alpha(1,4) linkage and therefore may regulate cell surface sialyl Lewis a expression and consequently regulates adhesive properties to E-selectin, cell proliferation and migration. Catalyzes the transfer of an L-fucose to 3'-sialyl-N-acetyllactosamine by an alpha(1,3) linkage, which allows the formation of sialyl-Lewis x structure and therefore may regulate the sialyl-Lewis x surface antigen expression and consequently adhesive properties to E-selectin. Prefers type 1 chain over type 2 acceptors. Type 1 tetrasaccharide is a better acceptor than type 1 disaccharide suggesting that a beta anomeric configuration of GlcNAc in the substrate is preferred. Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. |
| Protein Name | 3-Galactosyl-N-Acetylglucosaminide 4-Alpha-L-Fucosyltransferase Fut34-Galactosyl-N-Acetylglucosaminide 3-Alpha-L-FucosyltransferaseAlpha-3-Fucosyltransferase Fut3Blood Group Lewis Alpha-4-FucosyltransferaseLewis FtFucosyltransferase 3Fucosyltransferase IiiFuct-Iii |
| Database Links | Reactome: R-HSA-9037629Reactome: R-HSA-975578 |
| Cellular Localisation | Golgi ApparatusGolgi Stack MembraneSingle-Pass Type Ii Membrane ProteinMembrane-Bound Form In Trans Cisternae Of Golgi |
| Alternative ELISA Names | 3-Galactosyl-N-Acetylglucosaminide 4-Alpha-L-Fucosyltransferase Fut3 ELISA kit4-Galactosyl-N-Acetylglucosaminide 3-Alpha-L-Fucosyltransferase ELISA kitAlpha-3-Fucosyltransferase Fut3 ELISA kitBlood Group Lewis Alpha-4-Fucosyltransferase ELISA kitLewis Ft ELISA kitFucosyltransferase 3 ELISA kitFucosyltransferase Iii ELISA kitFuct-Iii ELISA kitFUT3 ELISA kitFT3B ELISA kitLE ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
